Home About Browse Search
Svenska


Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs

Domain, Guillaume and Hassan, Hiba Ali and Wydooghe, Eline and Pascottini, Osvaldo Bogado and Johannisson, Anders and Morrell, Jane and Nizanski, Wojciech and Van Soom, Ann (2022). Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs. Animals. 12 :6 , 714
[Research article]

[img] PDF
1MB

Abstract

Simple Summary Freezing dog semen is not always possible due to low quality sperm or poor survival during freezing. In order to make this assisted reproductive technique available to a larger number of dogs, this study investigated the benefit of selecting the best spermatozoa before freezing using single layer centrifugation (SLC). The results indicated that this technique was effective in separating spermatozoa according to their quality, although this resulted in losing some good quality spermatozoa. After thawing, spermatozoa centrifuged by SLC were of better quality than after standard centrifugation. However, spermatozoa from suboptimal quality semen did not survive freezing as well as spermatozoa from semen of optimal quality, even after SLC. Single layer centrifugation, therefore, makes it possible to obtain better quality spermatozoa after thawing but is not sufficient on its own to improve the inferior freezing ability of spermatozoa from suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile. This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) >= 80%, n = 9; suboptimal: NM between 60 and 79%, n = 9), were divided into two aliquots and subjected to standard centrifugation or SLC before cryopreservation. Motility, NM, membrane integrity, mitochondrial membrane potential (MMP), and DNA integrity were improved in fresh samples after SLC, regardless of semen quality, but at the expense of some good quality spermatozoa. After thawing, NM and membrane integrity were improved in SLC-selected semen in both semen qualities. Interestingly, MMP was also higher but only in optimal quality semen. Still, spermatozoa from suboptimal quality semen did not survive freezing to the same extent as spermatozoa from optimal quality semen, even after selecting superior spermatozoa. Semen selection with Canicoll is, therefore, an effective technique to isolate a subpopulation of high-quality spermatozoa and obtain sperm samples of better quality after thawing, but is not sufficient to improve the intrinsic inferior freezability of suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile.

Authors/Creators:Domain, Guillaume and Hassan, Hiba Ali and Wydooghe, Eline and Pascottini, Osvaldo Bogado and Johannisson, Anders and Morrell, Jane and Nizanski, Wojciech and Van Soom, Ann
Title:Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs
Series Name/Journal:Animals
Year of publishing :2022
Volume:12
Number:6
Article number:714
Number of Pages:13
Publisher:MDPI
ISSN:2076-2615
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:canine, fertility, semen selection, cryopreservation, semen quality
URN:NBN:urn:nbn:se:slu:epsilon-p-116784
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116784
Additional ID:
Type of IDID
DOI10.3390/ani12060714
Web of Science (WoS)000775584200001
ID Code:27644
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Apr 2022 11:25
Metadata Last Modified:28 Apr 2022 11:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits