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ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus

Jurca, Manuela and Sjoelander, Johan and Ibanez, Cristian and Matrosova, Anastasia and Johansson, Mikael and Kozarewa, Iwanka and Takata, Naoki and Bako, Laszlo and Webb, Alex A. R. and Israelsson Nordström, Maria and Eriksson, Maria E. (2022). ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus. Frontiers in Plant Science. 13 , 829121
[Research article]

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Abstract

Plants balance water availability with gas exchange and photosynthesis by controlling stomatal aperture. This control is regulated in part by the circadian clock, but it remains unclear how signalling pathways of daily rhythms are integrated into stress responses. The serine/threonine protein kinase OPEN STOMATA 1 (OST1) contributes to the regulation of stomatal closure via activation of S-type anion channels. OST1 also mediates gene regulation in response to ABA/drought stress. We show that ZEITLUPE (ZTL), a blue light photoreceptor and clock component, also regulates ABA-induced stomatal closure in Arabidopsis thaliana, establishing a link between clock and ABA-signalling pathways. ZTL sustains expression of OST1 and ABA-signalling genes. Stomatal closure in response to ABA is reduced in ztl mutants, which maintain wider stomatal apertures and show higher rates of gas exchange and water loss than wild-type plants. Detached rosette leaf assays revealed a stronger water loss phenotype in ztl-3, ost1-3 double mutants, indicating that ZTL and OST1 contributed synergistically to the control of stomatal aperture. Experimental studies of Populus sp., revealed that ZTL regulated the circadian clock and stomata, indicating ZTL function was similar in these trees and Arabidopsis. PSEUDO-RESPONSE REGULATOR 5 (PRR5), a known target of ZTL, affects ABA-induced responses, including stomatal regulation. Like ZTL, PRR5 interacted physically with OST1 and contributed to the integration of ABA responses with circadian clock signalling. This suggests a novel mechanism whereby the PRR proteins-which are expressed from dawn to dusk-interact with OST1 to mediate ABA-dependent plant responses to reduce water loss in time of stress.

Authors/Creators:Jurca, Manuela and Sjoelander, Johan and Ibanez, Cristian and Matrosova, Anastasia and Johansson, Mikael and Kozarewa, Iwanka and Takata, Naoki and Bako, Laszlo and Webb, Alex A. R. and Israelsson Nordström, Maria and Eriksson, Maria E.
Title:ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2022
Volume:13
Article number:829121
Number of Pages:18
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:abiotic stress, abscisic acid, circadian clock, stomatal closure, ZEITLUPE, OPEN STOMATA 1, PSEUDO-RESPONSE REGULATORs
URN:NBN:urn:nbn:se:slu:epsilon-p-116844
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116844
Additional ID:
Type of IDID
DOI10.3389/fpls.2022.829121
Web of Science (WoS)000773018600001
ID Code:27692
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:03 May 2022 13:25
Metadata Last Modified:03 May 2022 13:31

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