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Transformation systems, gene silencing and gene editing technologies in oomycetes

Ghimire, Bikal and Saraiva, Marcia and Andersen, Christian Benjamin and Gogoi, Anupam and Saleh, Mona and Zic, Nicola and van West, Pieter and Brurberg, May Bente (2022). Transformation systems, gene silencing and gene editing technologies in oomycetes. Fungal Biology Reviews. 40 , 37-52
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Abstract

Oomycetes are spore-forming eukaryotic microbes responsible for infections in animal and plant species worldwide, posing a threat to natural ecosystems, biodiversity and food security. Genomics and transcriptomics approaches, together with host interaction studies, give promising results towards better understanding of the infection mechanisms in oomycetes and their general biology. Significant development and progress in oomycetes genomic studies have been achieved over the past decades but further understanding of molecular processes, gene regulations and infection mechanisms are still needed. The use of molecular tools such as CRISPR/Cas and RNAi helped elucidate some of the molecular processes involved in host invasion and infection both in plant and animal pathogenic oomycetes. These methods provide an opportunity for accurate and detailed functional analysis involving various fields of studies such as genomics, epigenomics, proteomics, and interactomics. Functional gene characterisation is essential for filling the knowledge gaps in dynamic biological processes. However, every method has both advantages and limitations that should be considered before choosing the best method for investigating a particular research question. Here we review transformation systems, gene silencing and gene editing techniques in oomycetes, how they function, in which species and what are their main advantages and disadvantages.

Authors/Creators:Ghimire, Bikal and Saraiva, Marcia and Andersen, Christian Benjamin and Gogoi, Anupam and Saleh, Mona and Zic, Nicola and van West, Pieter and Brurberg, May Bente
Title:Transformation systems, gene silencing and gene editing technologies in oomycetes
Series Name/Journal:Fungal Biology Reviews
Year of publishing :2022
Volume:40
Page range:37-52
Number of Pages:16
ISSN:1749-4613
Language:English
Publication Type:Article Review/Survey
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
URN:NBN:urn:nbn:se:slu:epsilon-p-116699
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116699
Additional ID:
Type of IDID
DOI10.1016/j.fbr.2021.11.001
ID Code:27727
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:06 May 2022 12:03
Metadata Last Modified:06 May 2022 12:11

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