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Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism

Miao, Rui and Siao, Wei and Zhang, Na and Lei, Zuliang and Lin, Deshu and Bhalerao, Rishikesh P. and Lu, Congming and Xu, Weifeng (2022). Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism. International Journal of Molecular Sciences. 23 :7 , 3846
[Research article]

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Abstract

Root hydrotropism refers to root directional growth toward soil moisture. Cortical microtubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water.

Authors/Creators:Miao, Rui and Siao, Wei and Zhang, Na and Lei, Zuliang and Lin, Deshu and Bhalerao, Rishikesh P. and Lu, Congming and Xu, Weifeng
Title:Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2022
Volume:23
Number:7
Article number:3846
Number of Pages:11
Publisher:MDPI
ISSN:1661-6596
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:cortical microtubule arrays, oryzalin, KATANIN, abscisic acid, root hydrotropism
URN:NBN:urn:nbn:se:slu:epsilon-p-116928
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116928
Additional ID:
Type of IDID
DOI10.3390/ijms23073846
Web of Science (WoS)000780496100001
ID Code:27731
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:06 May 2022 13:10
Metadata Last Modified:06 May 2022 13:11

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