Home About Browse Search
Svenska


Genetic characterisation of an Iflavirus associated with a vomiting disease in the Indian tropical tasar silkworm, Antheraea mylitta

Ponnuvel, Kangayam M. and Rodrigues De Miranda, Joachim and Terenius, Olle and Li, Wenli and Ito, Katsuhiko and Khajje, Diksha and Shamitha, G. and Jagadish, Anupama and Dubey, Himanshu and Mishra, Rakesh K. (2022). Genetic characterisation of an Iflavirus associated with a vomiting disease in the Indian tropical tasar silkworm, Antheraea mylitta. Virus Research. 311 , 198703
[Research article]

[img] PDF
4MB

Abstract

Antheraea mylitta, the Tropical tasar silkworm, is frequently affected by a vomiting disease called Virosis by sericulturists although not confirmed being of viral origin. Based on the symptoms and the disease pattern, the causal agent is however suspected to be a virus. The condition involves a series of characteristic and progressive symptoms that generally culminates in the death of the larva. The disease is common in autumn season (SepOct), with widespread distribution causing severe damage to the tasar silk industry. The leads for this study were obtained from a transcript identified in the EST database in a different study, which matched the positive strand of Iflavirus, an RNA virus known to infect insects. In the present study the genome of this novel Iflavirus was characterised and the full length of the genome was found to be 9728 nucleotides long encoding for a single large open reading frame (ORF) with flanking NTR regions at 5 ' and 3 ' ends and a natural poly A tail at the 3 ' end. The ORF encoded structural proteins at the N-terminal end and non-structural proteins at the C-terminal end with a predicted 2967 amino acid long polyprotein. The structural proteins consisted of 4 proteins (VP1-VP4) and the non-structural proteins consisted of helicase, RNA-dependent RNA polymerase and 3C-protease. The virus is found in various tissues (midgut, fatbody, trachea, Malpighian tubules and silk gland) and also has a vertical route of transmission, i.e., from gravid females to the offspring. Based on the available data, the virus is a new member of Iflaviridae for which we propose the name Antheraea mylitta Iflavirus (AmIV).

Authors/Creators:Ponnuvel, Kangayam M. and Rodrigues De Miranda, Joachim and Terenius, Olle and Li, Wenli and Ito, Katsuhiko and Khajje, Diksha and Shamitha, G. and Jagadish, Anupama and Dubey, Himanshu and Mishra, Rakesh K.
Title:Genetic characterisation of an Iflavirus associated with a vomiting disease in the Indian tropical tasar silkworm, Antheraea mylitta
Series Name/Journal:Virus Research
Year of publishing :2022
Volume:311
Article number:198703
Number of Pages:11
Publisher:ELSEVIER
ISSN:0168-1702
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:Viral disease, Iflavirus, Antheraea mylitta, ORF, Genome
URN:NBN:urn:nbn:se:slu:epsilon-p-117064
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117064
Additional ID:
Type of IDID
DOI10.1016/j.virusres.2022.198703
Web of Science (WoS)000784295000009
ID Code:27798
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:12 May 2022 16:26
Metadata Last Modified:12 May 2022 16:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits