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Diel investments in metabolite production and consumption in a model microbial system

Uchimiya, Mario and Schroer, William and Olofsson, Malin and Edison, Arthur S. and Moran, Mary Ann (2021). Diel investments in metabolite production and consumption in a model microbial system. ISME Journal. 16 :5 , 1306-1317
[Research article]

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Abstract

Organic carbon transfer between surface ocean photosynthetic and heterotrophic microbes is a central but poorly understood process in the global carbon cycle. In a model community in which diatom extracellular release of organic molecules sustained growth of a co-cultured bacterium, we determined quantitative changes in the diatom endometabolome and the bacterial uptake transcriptome over two diel cycles. Of the nuclear magnetic resonance (NMR) peaks in the diatom endometabolites, 38% had diel patterns with noon or mid-afternoon maxima; the remaining either increased (36%) or decreased (26%) through time. Of the genes in the bacterial uptake transcriptome, 94% had a diel pattern with a noon maximum; the remaining decreased over time (6%). Eight diatom endometabolites identified with high confidence were matched to the bacterial genes mediating their utilization. Modeling of these coupled inventories with only diffusion-based phytoplankton extracellular release could not reproduce all the patterns. Addition of active release mechanisms for physiological balance and bacterial recognition significantly improved model performance. Estimates of phytoplankton extracellular release range from only a few percent to nearly half of annual net primary production. Improved understanding of the factors that influence metabolite release and consumption by surface ocean microbes will better constrain this globally significant carbon flux.

Authors/Creators:Uchimiya, Mario and Schroer, William and Olofsson, Malin and Edison, Arthur S. and Moran, Mary Ann
Title:Diel investments in metabolite production and consumption in a model microbial system
Series Name/Journal:ISME Journal
Year of publishing :2021
Volume:16
Number:5
Page range:1306-1317
Number of Pages:12
Publisher:SPRINGERNATURE
ISSN:1751-7362
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
URN:NBN:urn:nbn:se:slu:epsilon-p-115164
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115164
Additional ID:
Type of IDID
DOI10.1038/s41396-021-01172-w
Web of Science (WoS)000731204800002
Scopus2-s2.0-85121469040
ID Code:27818
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:14 May 2022 01:25
Metadata Last Modified:14 May 2022 05:01

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