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Identification of Genomic Regions and Sources for Wheat Blast Resistance through GWAS in Indian Wheat Genotypes

Phuke, Rahul M. and He, Xinyao and Juliana, Philomin and Kabir, Muhammad R. and Roy, Krishna K. and Marza, Felix and Roy, Chandan and Singh, Gyanendra P. and Chawade, Aakash and Joshi, Arun K. and Singh, Pawan K. (2022). Identification of Genomic Regions and Sources for Wheat Blast Resistance through GWAS in Indian Wheat Genotypes. Genes. 13 :4 , 596
[Research article]

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Abstract

Wheat blast (WB) is a devastating fungal disease that has recently spread to Bangladesh and poses a threat to the wheat production in India, which is the second-largest wheat producing country in the world. In this study, 350 Indian wheat genotypes were evaluated for WB resistance in 12 field experiments in three different locations, namely Jashore in Bangladesh and Quirusillas and Okinawa in Bolivia. Single nucleotide polymorphisms (SNPs) across the genome were obtained using DArTseq (R) technology, and 7554 filtered SNP markers were selected for a genome-wide association study (GWAS). All the three GWAS approaches used identified the 2NS translocation as the only major source of resistance, explaining up to 32% of the phenotypic variation. Additional marker-trait associations were located on chromosomes 2B, 3B, 4D, 5A and 7A, and the combined effect of three SNPs (2B_180938790, 7A_752501634 and 5A_618682953) showed better resistance, indicating their additive effects on WB resistance. Among the 298 bread wheat genotypes, 89 (29.9%) carried the 2NS translocation, the majority of which (60 genotypes) were CIMMYT introductions, and 29 were from India. The 2NS carriers with a grand mean WB index of 6.6 showed higher blast resistance compared to the non-2NS genotypes with a mean index of 46.5. Of the 52 durum wheats, only one genotype, HI 8819, had the 2NS translocation and was the most resistant, with a grand mean WB index of 0.93. Our study suggests that the 2NS translocation is the only major resistance source in the Indian wheat panel analysed and emphasizes the urgent need to identify novel non-2NS resistance sources and genomic regions.

Authors/Creators:Phuke, Rahul M. and He, Xinyao and Juliana, Philomin and Kabir, Muhammad R. and Roy, Krishna K. and Marza, Felix and Roy, Chandan and Singh, Gyanendra P. and Chawade, Aakash and Joshi, Arun K. and Singh, Pawan K.
Title:Identification of Genomic Regions and Sources for Wheat Blast Resistance through GWAS in Indian Wheat Genotypes
Series Name/Journal:Genes
Year of publishing :2022
Volume:13
Number:4
Article number:596
Number of Pages:16
Publisher:MDPI
ISSN:2073-4425
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Keywords:wheat blast, GWAS, 2NS translocation, resistance breeding
URN:NBN:urn:nbn:se:slu:epsilon-p-117020
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117020
Additional ID:
Type of IDID
DOI10.3390/genes13040596
Web of Science (WoS)000785242000001
ID Code:27889
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:16 May 2022 09:25
Metadata Last Modified:16 May 2022 09:31

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