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Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance

Sudhagar, Rajaprakasam and Priyanka, Shanmugavel and Chockalingam, Vanniarajan and Sendhilvel, Vaithiyanathan and Souframanien, Jegadeesan and Raja, Kalimuthu and Kanagarajan, Selvaraju (2022). Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance. Agronomy. 12 :4
[Research article]

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Abstract

Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3 to 4). The PM altered the chlorophyll fluorescence (a/b ratio), maturity duration, and yield attributing traits. The yield loss ranged from 4.55% to 72.66%. After affirming the resistance level, the resistant mutant (RM) with minimum yield loss (scale:0) and the susceptible mutant (SM) with maximum loss (scale:4) were used for metabolomic analysis through GC-MS. PM infection induced expression of 66 metabolites representing 32 functional classes. The number of unique classes in RM and SM was 13 and 11, respectively, while eight were common. A fold change in the common metabolites indicated an enhanced accumulation of amine, alcohol, and ester in RM. Along with pathogen-induced defensive metabolites, RM produced silane and fluorene, whose biological significance in disease resistance is unknown. Though SM expressed defence-related bio-molecules, it failed to yield better.

Authors/Creators:Sudhagar, Rajaprakasam and Priyanka, Shanmugavel and Chockalingam, Vanniarajan and Sendhilvel, Vaithiyanathan and Souframanien, Jegadeesan and Raja, Kalimuthu and Kanagarajan, Selvaraju
Title:Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance
Series Name/Journal:Agronomy
Year of publishing :2022
Volume:12
Number:4
Number of Pages:14
Publisher:MDPI
ISSN:2073-4395
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:E. polygoni, GC-MS, horse gram, metabolomics
URN:NBN:urn:nbn:se:slu:epsilon-p-117022
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117022
Additional ID:
Type of IDID
DOI10.3390/agronomy12040800
Web of Science (WoS)000785269300001
ID Code:27913
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:17 May 2022 07:25
Metadata Last Modified:17 May 2022 07:31

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