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The influence of sucrose on soil nitrogen availability - A root exudate simulation using microdialysis

Buckley, Scott and Brackin, Richard and Näsholm, Torgny and Schmidt, Susanne and Jämtgård, Sandra (2022). The influence of sucrose on soil nitrogen availability - A root exudate simulation using microdialysis. Geoderma. 409 , 115645
[Research article]

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Abstract

Root exudates are thought to promote nitrogen (N) availability via rhizosphere interactions, but empirical evidence is difficult to obtain given the scale and temporary nature of these processes. Microdialysis has potential to simulate root exudation patterns and quantify the effects on N availability simultaneously, but this has so far not been attempted. In a conceptual root exudation study, we used sucrose as a simple C source to investigate if microdialysis could detect the effects of continuous localised C supply on soil inorganic N fluxes. Through retrodialysis we released sucrose and simultaneously monitored diffusive soil N fluxes over one week, followed by a further seven days without sucrose. Based on current understanding of rhizosphere N dynamics, we hypothesised that N fluxes are inversely related to sucrose release, and upon ceasing release, N fluxes would increase. Using a 5 mM sucrose perfusate, C releases resulted in decreased N fluxes, but contrary to our hypothesis, N fluxes did not increase after ceasing sucrose release (c.f. control soil). Diffusive sucrose efflux from microdialysis probes increased in soils amended with N-rich litter suggesting that microbial activity and associated sucrose consumption altered sucrose concentration gradients. The fluxes of sucrose breakdown products glucose and fructose were greatest in litter treatments receiving sucrose, indicative of increased invertase activity in the presence of fresh organic matter. In the short term (days), sucrose release did not prompt an increase in inorganic N availability, possibly because of stimulated microbial growth and increased N demand under C-rich conditions. Our study confirms that microdialysis allows time-sensitive insight into the dynamic interactions of carbon and N in the rhizosphere.

Authors/Creators:Buckley, Scott and Brackin, Richard and Näsholm, Torgny and Schmidt, Susanne and Jämtgård, Sandra
Title:The influence of sucrose on soil nitrogen availability - A root exudate simulation using microdialysis
Series Name/Journal:Geoderma
Year of publishing :2022
Volume:409
Article number:115645
Number of Pages:10
Publisher:ELSEVIER
ISSN:0016-7061
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Soil Science
Keywords:Microdialysis, Root exudation, Soil nitrogen, Litter decomposition, Soil carbon, Rhizosphere interactions
URN:NBN:urn:nbn:se:slu:epsilon-p-117095
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117095
Additional ID:
Type of IDID
DOI10.1016/j.geoderma.2021.115645
Web of Science (WoS)000788127100001
ID Code:27955
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Ecology and Management
Deposited By: SLUpub Connector
Deposited On:19 May 2022 11:25
Metadata Last Modified:19 May 2022 11:31

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