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Uncovering antimicrobial resistance in three agricultural biogas plants using plant-based substrates

Sun, He and Schnürer, Anna and Müller, Bettina and Mößnang, Bettina and Lebuhn, Michael and Makarewicz, Oliwia (2022). Uncovering antimicrobial resistance in three agricultural biogas plants using plant-based substrates. Science of the Total Environment. 829 , 154556
[Research article]

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Abstract

Antimicrobial resistance (AMR) is becoming an increasing global concern and the anaerobic digestion (AD) process represents a potential transmission route when digestates are used as fertilizing agents. AMR contaminants, e.g. antibiotic-resistant bacteria (ARB) and plasmid-mediated antibiotic resistance genes (ARGs) have been found in different substrates and AD systems, but not yet been investigated in plant-based substrates. AMR transfer from soils to vegetable microbiomes has been observed, and thus crop material potentially represents a so far neglected AMR load in agricultural AD processes, contributing to AMR spread. In order to test this hypothesis, this study examined the AMR situation throughout the process of three biogas plants using plant-based substrates only, or a mixture of plant-based and manure substrates. The evaluation included a combination of culture-independent and -dependent methods, i.e., identification of ARGs, plasmids, and pathogenic bacteria by DNA arrays, and phylogenetic classification of bacterial isolates and their phenotypic resistance pattern. To our knowledge, this is the first study on AMR in plant based substrates and the corresponding biogas plant. The results showed that the bacterial community isolated from the investigated substrates and the AD processing facilities were mainly Gram-positive Bacillus spp. Apart from Pantoea agglomerans, no other Gram-negative species were found, either by bacteria culturing or by DNA typing array. In contrast, the presence of ARGs and plasmids clearly indicated the existence of Gram-negative pathogenic bacteria, in both substrate and AD process. Compared with substrates, digestates had lower levels of ARGs, plasmids, and culturable ARB. Thus, digestate could pose a lower risk of spreading AMR than substrates per se. In conclusion, plant-based substrates are associated with AMR, including culturable Gram-positive ARB and Gram-negative pathogenic bacteria associated ARGs and plasmids. Thus, the AMR load from plant-based substrates should be taken into consideration in agricultural biogas processing.

Authors/Creators:Sun, He and Schnürer, Anna and Müller, Bettina and Mößnang, Bettina and Lebuhn, Michael and Makarewicz, Oliwia
Title:Uncovering antimicrobial resistance in three agricultural biogas plants using plant-based substrates
Series Name/Journal:Science of the Total Environment
Year of publishing :2022
Volume:829
Article number:154556
Number of Pages:10
Publisher:ELSEVIER
ISSN:0048-9697
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Environmental Sciences related to Agriculture and Land-use
Keywords:Antimicrobial resistance, Agricultural biogas plant, Substrate, Digestate, Antibiotic-resistant bacteria, Antibiotic resistance genes
URN:NBN:urn:nbn:se:slu:epsilon-p-117138
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117138
Additional ID:
Type of IDID
DOI10.1016/j.scitotenv.2022.154556
Web of Science (WoS)000791200600015
ID Code:27958
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:19 May 2022 14:25
Metadata Last Modified:19 May 2022 14:31

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