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Metabolomics reveals changes in metabolite profiles due to growth and metamorphosis during the ontogeny of the northern damselfly

Spath, Jana and Brodin, Tomas and McCallum, Erin and Cerveny, Daniel and Fick, Jerker and Nording, Malin L. (2022). Metabolomics reveals changes in metabolite profiles due to growth and metamorphosis during the ontogeny of the northern damselfly. Journal of Insect Physiology. 136 , 104341
[Research article]

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Abstract

Many insects have complex life cycles where a drastic ontogenetic change happens between the larval stages and the adult stage, i.e. metamorphosis. Damselflies (order Odonata, suborder Zygoptera) are widely distributed and ecologically important semi-aquatic insects with a complex life cycle. Phenotypic changes over damselfly ontogeny have been documented, however, if and how metabolite profiles are also changing is currently unknown. Here we used a metabolomics methodology to gain insights into the metabolic changes during the life cycle of the Northern damselfly (Coenagrion hastulatum). Hatchlings of wild-caught damselflies were reared in the laboratory and metabolomics analyses using liquid chromatography and gas chromatography coupled to mass spectrometry were carried out at three larval stages and on adult damselflies. Additionally, a subset of larvae was exposed to wastewater effluent to assess how metabolite profiles responded to an environmental stressor. A total of 212 compounds belonging to several classes (e.g. amino acids, fatty acids, sugars) were annotated. Across metamorphosis, we found that damselflies shifted from protein catabolism to lipid catabolism. Wastewater effluent exposure resulted in ontogenetic stage-dependent changes of individual metabolites, but not to a marked extent. Overall, our study is one of the first to describe changes of metabolite profiles during ontogeny of an insect, and it provides a first step towards a greater understanding of the physiological changes occurring during general insect-but especially damselfly-ontogeny.

Authors/Creators:Spath, Jana and Brodin, Tomas and McCallum, Erin and Cerveny, Daniel and Fick, Jerker and Nording, Malin L.
Title:Metabolomics reveals changes in metabolite profiles due to growth and metamorphosis during the ontogeny of the northern damselfly
Series Name/Journal:Journal of Insect Physiology
Year of publishing :2022
Volume:136
Article number:104341
Number of Pages:8
Publisher:PERGAMON-ELSEVIER SCIENCE LTD
ISSN:0022-1910
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology
Keywords:Semi-aquatic invertebrate, Odonata, Complex life cycle, Mass spectrometry, Chromatography, Wastewater treatment plant effluent
URN:NBN:urn:nbn:se:slu:epsilon-p-117103
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117103
Additional ID:
Type of IDID
DOI10.1016/j.jinsphys.2021.104341
Web of Science (WoS)000787580200009
ID Code:27967
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Wildlife, Fish and Environmental Studies
Deposited By: SLUpub Connector
Deposited On:20 May 2022 12:25
Metadata Last Modified:20 May 2022 12:31

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