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Seed inoculation with antagonistic bacteria limits occurrence of E. coli O157:H7gfp+ on baby spinach leaves

Karlsson, Maria and Uhlig, Elisabeth and Håkansson, Åsa and Alsanius, Beatrix (2022). Seed inoculation with antagonistic bacteria limits occurrence of E. coli O157:H7gfp+ on baby spinach leaves. BMC Microbiology. 22 , 131
[Research article]

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Abstract

Backround During the last decades, outbreaks of foodborne illnesses have increasingly been linked to fresh and/or minimally processed fruit and vegetables. Enterohemorrhagic Escherichia coli was the causal agent for major outbreaks in Europe with leafy green vegetables and sprouts. To improve food safety, microbial antagonism has received attention during recent years and could be one of the solution to prevent contamination of food borne pathogens on fresh produce. Here we investigate the antagonistic effect of three bacterial strains (Pseudomonas orientalis, P. flavescens and Rhodococcus sp.) isolated from spinach leaves against E. coli O157:H7gfp + under laboratory and greenhouse conditions. Results Our results shows that significantly less culturable E.coli O157:H7gfp + were retrieved from the spinach canopy subjected to antagonist seed treatment than canopy inoculation. Seeds inoculated with Rhodococcus sp. significantly reduced growth of E. coli O157:H7gfp + compared with the other antagonists. The result from the in vitro study shows a significant reduction of growth of E. coli O157:H7gfp+, but only after 44 h when E. coli O157:H7gfp + was propagated in a mixture of spent media from all three antagonists. Conclusions The antagonistic effect on phyllospheric E.coli O157:H7gfp + observed after seed inoculation with Rhodococcus sp. might be an indication of induced resistance mechanism in the crop. In addition, there was a small reduction of culturable E.coli O157:H7gfp + when propagated in spent media from all three antagonists. Nutritional conditions rather than metabolites formed by the three chosen organisms appear to be critical for controlling E. coli O157:H7gfp+.

Authors/Creators:Karlsson, Maria and Uhlig, Elisabeth and Håkansson, Åsa and Alsanius, Beatrix
Title:Seed inoculation with antagonistic bacteria limits occurrence of E. coli O157:H7gfp+ on baby spinach leaves
Series Name/Journal:BMC Microbiology
Year of publishing :2022
Volume:22
Article number:131
Number of Pages:7
Associated Programs and Other Stakeholders:Z - SLU - Library > Odla mera
ISSN:1471-2180
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Keywords:Bacterial antagonist, E. coli O157:H7, Pseudomonas favescens, Pseudomonas orientalis, Rhodococcus sp., Spinach
URN:NBN:urn:nbn:se:slu:epsilon-p-117159
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117159
Additional ID:
Type of IDID
DOI10.1186/s12866-022-02550-w
ID Code:27969
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
Deposited By: SLUpub Connector
Deposited On:20 May 2022 13:25
Metadata Last Modified:21 May 2022 04:00

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