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Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis

Oliver Velasco, Cecilia and Martinez Arias, German (2022). Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reproduction. 35 :2 , 153-160
[Research article]

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Abstract

Meiosis is a specialized cell division that is key for reproduction and genetic diversity in sexually reproducing plants. Recently, different RNA silencing pathways have been proposed to carry a specific activity during meiosis, but the pathways involved during this process remain unclear. Here, we explored the subcellular localization of different ARGONAUTE (AGO) proteins, the main effectors of RNA silencing, during male meiosis in Arabidopsis thaliana using immunolocalizations with commercially available antibodies. We detected the presence of AGO proteins associated with posttranscriptional gene silencing (AGO1, 2, and 5) in the cytoplasm and the nucleus, while AGOs associated with transcriptional gene silencing (AGO4 and 9) localized exclusively in the nucleus. These results indicate that the localization of different AGOs correlates with their predicted roles at the transcriptional and posttranscriptional levels and provide an overview of their timing and potential role during meiosis.

Authors/Creators:Oliver Velasco, Cecilia and Martinez Arias, German
Title:Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis
Series Name/Journal:Plant Reproduction
Year of publishing :2022
Volume:35
Number:2
Page range:153-160
Number of Pages:8
Publisher:SPRINGER
ISSN:2194-7953
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Meiosis, RNA silencing, Argonaute, Small RNAs, Pollen, Epigenetics
URN:NBN:urn:nbn:se:slu:epsilon-p-114528
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114528
Additional ID:
Type of IDID
DOI10.1007/s00497-021-00434-z
Web of Science (WoS)000721638000001
Scopus2-s2.0-85119667455
ID Code:27973
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:23 May 2022 13:25
Metadata Last Modified:23 May 2022 13:31

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