Samaras, Anastasios and Kamou, Nathalie and Tzelepis, Georgios and Karamanoli, Katerina and Menkissoglu-Spiroudi, Urania and Karaoglanidis, George S.
(2022).
Root Transcriptional and Metabolic Dynamics Induced by the Plant Growth Promoting Rhizobacterium (PGPR) Bacillus subtilis Mbi600 on Cucumber Plants.
Plants. 11
:9
, 1218
[Research article]
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Abstract
Bacillus subtilis MBI600 is a commercialized plant growth-promoting bacterial species used as a biocontrol agent in many crops, controlling various plant pathogens via direct or indirect mechanisms. In the present study, a detailed transcriptomic analysis of cucumber roots upon response to the Bs MBI600 strain is provided. Differentially expressed genes (DEGs) analysis showed altered gene expression in more than 1000 genes at 24 and 48 h post-application of Bs MBI600. Bs MBI600 induces genes involved in ISR and SAR signaling. In addition, genes involved in phytohormone production and nutrient availability showed an upregulation pattern, justifying the plant growth promotion. Biocontrol ability of Bs MBI600 seems also to be related to the activation of defense-related genes, such as peroxidase, endo-1,3(4)-beta-glucanase, PR-4, and thaumatin-like. Moreover, KEGG enriched results showed that differentially expressed genes were classified into biocontrol-related pathways. To further investigate the plant's response to the presence of PGPR, a profile of polar metabolites of cucumber treated with Bs MBI600 was performed and compared to that of untreated plants. The results of the current study gave insights into the mechanisms deployed by this biocontrol agent to promote plant resistance, helping to understand the molecular interactions in this system.
| Authors/Creators: | Samaras, Anastasios and Kamou, Nathalie and Tzelepis, Georgios and Karamanoli, Katerina and Menkissoglu-Spiroudi, Urania and Karaoglanidis, George S. | ||||||
|---|---|---|---|---|---|---|---|
| Title: | Root Transcriptional and Metabolic Dynamics Induced by the Plant Growth Promoting Rhizobacterium (PGPR) Bacillus subtilis Mbi600 on Cucumber Plants | ||||||
| Series Name/Journal: | Plants | ||||||
| Year of publishing : | 2022 | ||||||
| Volume: | 11 | ||||||
| Number: | 9 | ||||||
| Article number: | 1218 | ||||||
| Number of Pages: | 17 | ||||||
| Publisher: | MDPI | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany | ||||||
| Keywords: | Bacillus MBI600, transcriptomic analysis, metabolomic analysis, defense-related proteins, plant growth promotion | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-117269 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117269 | ||||||
| Additional ID: |
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| ID Code: | 28160 | ||||||
| Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
| Department: | (NL, NJ) > Dept. of Forest Mycology and Plant Pathology (S) > Dept. of Forest Mycology and Plant Pathology | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 01 Jun 2022 10:33 | ||||||
| Metadata Last Modified: | 01 Jun 2022 10:41 |
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