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The equine IGF genes : structural and transcriptional features

Otte, Kerstin (1997). The equine IGF genes : structural and transcriptional features. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae. Veterinaria, 1401-6257
ISBN 91-576-5418-2
[Doctoral thesis]

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Abstract

Insulin-like growth factors 1 and 2 (IGF1 and IGF2) are small mitogenic peptides. IGF! is mainly expressed in adult life, whereas IGF2 is one of the most ubiquitous growth factors in the mammalian embryo, where it plays an important role in the regulation of fetal growth. The encoding genes have been characterised in a number ofspecies and show a complex structural organisation and regulation of expression. Furthermore, IGF2 it is subject to epigenetic modification and genomic imprinting.The present study aimed at the basic structural and functional characterisation ofthe equine IGF1 and IGF2 genes.cDNAs for both equine IGF1 and IGF2 were cloned and showed high sequence homology when compared to other species. Even more conserved is the deduced amino add sequence of both peptides. The equine IGF2 gene was cloned and characterised. Sequence comparison revealed three non-coding leader exons and three coding exons. Downstream of the polyadenylation site a dinucleotide repeat sequence was identified. A novel structural element, an inverted repeat, is predicted. It is conserved between species and located in a region which is differentially methylated in the human and mouse genes and possibly involved in the imprinting mechanism. The inverted repeat acquires a stem-loop structure with a hybrid A/B-DNA conformation in the stem area and binds a specific, methylation sensitive protein which is developmentally regulated.A number oftranscripts were detected for both equine IGF1 and IGF2 genes. In the IGF2 gene, two promoters (P2 and P3) were shown to be active in fetal tissues and one promoter (P3) was found to be active in adult tissues. This represents a transcriptional pattern different from that in humans or rodents. Furthermore, IGF2 transcripts were located at tissue level using in situ hybridisation.The equine IGF2 gene was physically mapped to ECA 12ql3 and homoeology of this chromosome to HSA11 was revealed.

Authors/Creators:Otte, Kerstin
Title:The equine IGF genes : structural and transcriptional features
Series Name/Journal:Acta Universitatis Agriculturae Sueciae. Veterinaria
Year of publishing :1997
Number:24
Number of Pages:54
Publisher:Swedish University of Agricultural Sciences
ISBN for printed version:91-576-5418-2
ISSN:1401-6257
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:insulin-like growth factor, equine, cloning, transcription, physical localisation, stem-loop, imprinting
URN:NBN:urn:nbn:se:slu:epsilon-p-117383
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117383
ID Code:28319
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Institutionen för patologi
Deposited By: SLUpub Connector
Deposited On:13 Jun 2022 08:43
Metadata Last Modified:13 Jun 2022 09:07

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