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Genome mapping of quantitative trait loci in Salix with an emphasis on freezing resistance

Tsarouhas, Vasilios (2002). Genome mapping of quantitative trait loci in Salix with an emphasis on freezing resistance. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae. Agraria, 1401-6249
ISBN 91-576-6164-2
[Doctoral thesis]

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Abstract

The current advancement of biotechnology could provide a better understanding of the genetic control and the molecular basis of quantitative traits in plants. The present thesis focuses on the identification of genes affecting freezing resistance and phenological traits. Two genetic maps were constructed for two interspecific Salix families. The first map was based on a backcross (n = 87) ofthe male clone “Björn” (Salix viminalis x Salix schwerinii) with the female clone “78183” (Salix viminalis) and composed of 325 AFLP and 25 RFLP loci, the latter mainly derived from the Populus genome. The second map consisted of 433 AFLPs and it was based on an F? family (n = 92) with grandparents being a frost susceptible female clone (“Jorunn”; S. viminalis) and a frost resistant male clone (“SW901290”; 5. dasyclados like). The average length ofthe maps and the genome coverage were 2483 cM and 72% respectively, while the average distance between loci was about 11 cM.Using the genetic map of the backcross family, nineteen quantitative trait loci (QTL) were identified; eleven for growth-related traits and eight for the timing of bud flush. The estimated magnitude of the QTL effect ranged from 12 to 24% of the total phenotypic variance. One QTL for height growth, one for diameter and one for the height:diameter ratio, were found clustered in the same marker interval. One QTL associated with indoor bud flushing coincided with a QTL controlling timing of bud flush in the field. Little evidence was found for QTL stability in height growth over 3 consecutive years.Ten genomic regions controlling freezing resistance and nine affecting phenological traits were identified during cold acclimation ofthe F? family. The magnitude ofthe phenotypic variation explained by each freezing resistance locus varied over acclimation time (3 - 45%) and there was no time point at which all the QTL could be detected. The single QTL detected for non-acclimated freezing resistance did not reach significance at any time during cold acclimation suggesting an independent relationship between non-acclimated and acclimated freezing resistance in Salix. The determination of QTL position on the map suggested the partial involvement of a common set of genes for autumn freezing resistance and phenology traits. Of the 14 QTL controlling autumn freezing resistance and phenological traits at the indoor experiment, six (43%) were associated with autumn phenology traits i.e. total height increment, dry-to-fresh weight ratio and number of new leaves, as measured in the field. A major locus with multi-trait association in both indoor and outdoor experiments was detected. The presence of genes with large effect for growth and adaptation traits suggests that a marker assisted selection breeding scheme could accelerate the breeding process in Salix.

Authors/Creators:Tsarouhas, Vasilios
Title:Genome mapping of quantitative trait loci in Salix with an emphasis on freezing resistance
Series Name/Journal:Acta Universitatis Agriculturae Sueciae. Agraria
Year of publishing :2002
Number:327
Number of Pages:62
Publisher:Swedish University of Agricultural Sciences
ISBN for printed version:91-576-6164-2
ISSN:1401-6249
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:genetic mapping, QTL mapping, genetic markers, freezing resistance, phenology traits, growth traits, Salix
URN:NBN:urn:nbn:se:slu:epsilon-p-117503
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117503
ID Code:28390
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:20 Jun 2022 09:26
Metadata Last Modified:20 Jun 2022 09:44

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