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KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport

Hamon-Josse, Maxime and Villaecija-Aguilar, Jose Antonio and Ljung, Karin and Leyser, Ottoline and Gutjahr, Caroline and Bennett, Tom (2022). KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport. New Phytologist. 235 :1 , 126-140
[Research article]

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Abstract

Photomorphogenic remodelling of seedling growth is a key developmental transition in the plant life cycle. The alpha/beta-hydrolase signalling protein KARRIKIN-INSENSITIVE2 (KAI2), a close homologue of the strigolactone receptor DWARF14 (D14), is involved in this process, but it is unclear how the effects of KAI2 on development are mediated. Here, using a combination of physiological, pharmacological, genetic and imaging approaches in Arabidopsis thaliana (Heynh.) we show that kai2 phenotypes arise because of a failure to downregulate auxin transport from the seedling shoot apex towards the root system, rather than a failure to respond to light per se. We demonstrate that KAI2 controls the light-induced remodelling of the PIN-mediated auxin transport system in seedlings, promoting a reduction in PIN7 abundance in older tissues, and an increase of PIN1/PIN2 abundance in the root meristem. We show that removing PIN3, PIN4 and PIN7 from kai2 mutants, or pharmacological inhibition of auxin transport and synthesis, is sufficient to suppress most kai2 seedling phenotypes. We conclude that KAI2 regulates seedling morphogenesis by its effects on the auxin transport system. We propose that KAI2 is not required for the light-mediated changes in PIN gene expression but is required for the appropriate changes in PIN protein abundance within cells.

Authors/Creators:Hamon-Josse, Maxime and Villaecija-Aguilar, Jose Antonio and Ljung, Karin and Leyser, Ottoline and Gutjahr, Caroline and Bennett, Tom
Title:KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport
Series Name/Journal:New Phytologist
Year of publishing :2022
Volume:235
Number:1
Page range:126-140
Number of Pages:15
Publisher:WILEY
ISSN:0028-646X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:Arabidopsis, auxin, auxin transport, KAI2 signalling, light signalling, PIN proteins, seedling development
URN:NBN:urn:nbn:se:slu:epsilon-p-116861
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116861
Additional ID:
Type of IDID
DOI10.1111/nph.18110
Web of Science (WoS)000780402200001
Scopus2-s2.0-85127628471
ID Code:28395
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:20 Jun 2022 14:26
Metadata Last Modified:20 Jun 2022 14:31

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