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Influence of cysteine, serine, sulfate, and sulfide on anaerobic conversion of unsaturated long-chain fatty acid, oleate, to methane

Yekta, Sepehr Shakeri and Elreedy, Ahmed and Liu, Tong and Hedenstrom, Mattias and Isaksson, Simon and Fujii, Manabu and Schnürer, Anna and Schnurer, Anna (2022). Influence of cysteine, serine, sulfate, and sulfide on anaerobic conversion of unsaturated long-chain fatty acid, oleate, to methane. Science of the Total Environment. 817 , 152967
[Research article]

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Abstract

This study aims to elucidate the role of sulfide and its precursors in anaerobic digestion (i.e., cysteine, representing sulfur-containing amino acids, and sulfate) on microbial oleate conversion to methane. Serine, with a similar structure to cysteine but with a hydroxyl group instead of a thiol, was included as a control to assess potential effects on methane formation that were not related to sulfur functionalities. The results showed that copresence of sulfide and oleate in anaerobic batch assays accelerated the methane formation compared to assays with only oleate and mitigated negative effect on methane formation caused by increased sulfide level. Nuclear magnetic resonance spectroscopy of sulfide exposed oleate suggested that sulfide reaction with oleate double bonds likely contributed to negation of the negative effect on the methanogenic activity. Methane formation from oleate was also accelerated in the presence of cysteine or serine, while sulfate decreased the cumulative methane formation from oleate. Neither cysteine nor serine was converted to methane, and their accelerating effects was associated to different mechanisms due to establishment of microbial communities with different structures, as evidenced by high-throughput sequencing of 16S rRNA gene. These outcomes contribute with new knowledge to develop strategies for optimum use of sulfur-and lipid-rich wastes in anaerobic digestion processes.

Authors/Creators:Yekta, Sepehr Shakeri and Elreedy, Ahmed and Liu, Tong and Hedenstrom, Mattias and Isaksson, Simon and Fujii, Manabu and Schnürer, Anna and Schnurer, Anna
Title:Influence of cysteine, serine, sulfate, and sulfide on anaerobic conversion of unsaturated long-chain fatty acid, oleate, to methane
Series Name/Journal:Science of the Total Environment
Year of publishing :2022
Volume:817
Article number:152967
Number of Pages:7
Publisher:ELSEVIER
ISSN:0048-9697
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Sulfur, Amino acids, Oleate, beta-Oxidation, Anaerobic digestion, Methane
URN:NBN:urn:nbn:se:slu:epsilon-p-117354
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117354
Additional ID:
Type of IDID
DOI10.1016/j.scitotenv.2022.152967
Web of Science (WoS)000793348100002
ID Code:28470
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:18 Aug 2022 14:27
Metadata Last Modified:18 Aug 2022 14:31

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