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No evidence of uptake or propagation of reindeer CWD prions in environmentally exposed sheep

Harpaz, Erez and Salvesen, Oyvind and Rauset, Geir Rune and Mahmood, Aqsa and Tran, Linh and Ytrehus, Bjornar and Ytrehus, Björnar and Benestad, Sylvie Lafond and Tranulis, Michael Andreas and Espenes, Arild and Ersdal, Cecilie (2022). No evidence of uptake or propagation of reindeer CWD prions in environmentally exposed sheep. Acta Veterinaria Scandinavica. 64 , 13
[Research article]

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Abstract

Background Chronic wasting disease (CWD) is a prion disease of cervids first reported in North America in the 1960s. In Europe, CWD was first diagnosed in 2016 in a wild reindeer in Norway. Detection of two more cases in the same mountain area led to the complete culling of this partially confined reindeer population of about 2400 animals. A total of 19 CWD positive animals were identified. The affected area is extensively used for the grazing of sheep during summers. There are many mineral licks intended for sheep in the area, but these have also been used by reindeer. This overlap in area use raised concerns for cross-species prion transmission between reindeer and sheep. In this study, we have used global positioning system (GPS) data from sheep and reindeer, including tracking one of the CWD positive reindeer, to investigate spatial and time-relevant overlaps between these two species. Since prions can accumulate in lymphoid follicles following oral uptake, samples of gut-associated lymphoid tissue (GALT) from 425 lambs and 78 adult sheep, which had grazed in the region during the relevant timeframe, were analyzed for the presence of prions. The recto-anal mucosa associated lymphoid tissue (RAMALT) from all the animals were examined by histology, immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA), and the ileal Peyer's patch (IPP) from a subsample of 37 lambs were examined by histology and IHC, for the detection of prions. Results GPS data showed an overlap in area use between the infected reindeer herd and the sheep. In addition, the GPS positions of an infected reindeer and some of the sampled sheep showed temporospatial overlap. No prions were detected in the GALT of the investigated sheep even though the mean lymphoid follicle number in RAMALT and IPP samples were high. Conclusion The absence of prions in the GALT of sheep that have shared pasture with CWD-infected reindeer, may suggest that transmission of this novel CWD strain to sheep does not easily occur under the conditions found in these mountains. We document that the lymphoid follicle rich RAMALT could be a useful tool to screen for prions in sheep.

Authors/Creators:Harpaz, Erez and Salvesen, Oyvind and Rauset, Geir Rune and Mahmood, Aqsa and Tran, Linh and Ytrehus, Bjornar and Ytrehus, Björnar and Benestad, Sylvie Lafond and Tranulis, Michael Andreas and Espenes, Arild and Ersdal, Cecilie
Title:No evidence of uptake or propagation of reindeer CWD prions in environmentally exposed sheep
Series Name/Journal:Acta Veterinaria Scandinavica
Year of publishing :2022
Volume:64
Article number:13
Number of Pages:11
Publisher:BMC
ISSN:0044-605X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:ELISA, Immunohistochemistry, Nordfjella, Norway, Prion, RAMALT
URN:NBN:urn:nbn:se:slu:epsilon-p-117554
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117554
Additional ID:
Type of IDID
DOI10.1186/s13028-022-00632-3
Web of Science (WoS)000806794100001
ID Code:28472
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:18 Aug 2022 14:54
Metadata Last Modified:12 Sep 2022 21:19

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