Home About Browse Search
Svenska


Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources

Cheng, George and Gabler, Florian and Pizzul, Leticia and Olsson, Henrik and Nordberg, Ake and Nordberg, Åke and Schnürer, Anna (2022). Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources. Applied Microbiology and Biotechnology. 106 , 5317-5333
[Research article]

[img] PDF
1MB

Abstract

Microbial community development within an anaerobic trickle bed reactor (TBR) during methanation of syngas (56% H-2, 30% CO, 14% CO2) was investigated using three different nutrient media: defined nutrient medium (241 days), diluted digestate from a thermophilic co-digestion plant operating with food waste (200 days) and reject water from dewatered digested sewage sludge at a wastewater treatment plant (220 days). Different TBR operating periods showed slightly different performance that was not clearly linked to the nutrient medium, as all proved suitable for the methanation process. During operation, maximum syngas load was 5.33 L per L packed bed volume (pbv) & day and methane (CH4) production was 1.26 L CH4/L-pbv/d. Microbial community analysis with Illumina Miseq targeting 16S rDNA revealed high relative abundance (20-40%) of several potential syngas and acetate consumers within the genera Sporomusa, Spirochaetaceae, Rikenellaceae and Acetobacterium during the process. These were the dominant taxa except in a period with high flow rate of digestate from the food waste plant. The dominant methanogen in all periods was a member of the genus Methanobacterium, while Methanosarcina was also observed in the carrier community. As in reactor effluent, the dominant bacterial genus in the carrier was Sporomusa. These results show that syngas methanation in TBR can proceed well with different nutrient sources, including undefined medium of different origins. Moreover, the dominant syngas community remained the same over time even when non-sterilised digestates were used as nutrient medium.

Authors/Creators:Cheng, George and Gabler, Florian and Pizzul, Leticia and Olsson, Henrik and Nordberg, Ake and Nordberg, Åke and Schnürer, Anna
Title:Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources
Series Name/Journal:Applied Microbiology and Biotechnology
Year of publishing :2022
Volume:106
Page range:5317-5333
Number of Pages:17
Publisher:SPRINGER
ISSN:0175-7598
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Methanation, Syngas, Microbial community, Sporomusa, Methanobacterium, Trickling bed reactor
URN:NBN:urn:nbn:se:slu:epsilon-p-118429
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118429
Additional ID:
Type of IDID
DOI10.1007/s00253-022-12035-5
Web of Science (WoS)000825379600001
ID Code:28515
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
(NL, NJ) > Dept. of Energy and Technology
Deposited By: SLUpub Connector
Deposited On:22 Aug 2022 13:28
Metadata Last Modified:22 Aug 2022 13:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits