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Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli

Diaz, Silvia and Aguilera, Angeles and de Figueras, Carolina G. and de Francisco, Patricia and Olsson, Sanna and Puente Sanchez, Fernando and Eduardo Gonzalez-Pastor, Jose (2022). Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli. International journal of environmental research and public health. 19 :13 , 7692
[Research article]

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Abstract

Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5 alpha, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.

Authors/Creators:Diaz, Silvia and Aguilera, Angeles and de Figueras, Carolina G. and de Francisco, Patricia and Olsson, Sanna and Puente Sanchez, Fernando and Eduardo Gonzalez-Pastor, Jose
Title:Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
Series Name/Journal:International journal of environmental research and public health
Year of publishing :2022
Volume:19
Number:13
Article number:7692
Number of Pages:21
Publisher:MDPI
ISSN:1661-7827
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:phytochelatins, heavy metal resistance, stress response, oxidative stress, bioaccumulation
URN:NBN:urn:nbn:se:slu:epsilon-p-118469
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118469
Additional ID:
Type of IDID
DOI10.3390/ijerph19137692
Web of Science (WoS)000825563200001
ID Code:28548
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:25 Aug 2022 09:28
Metadata Last Modified:25 Aug 2022 09:31

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