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Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants

Löfgren, Maria and Ekman, Stina and Ekholm, Josefine and Engstrom, Mona and Fjordbakk, Cathrine T. and Svala, Emilia and Holm Forsström, Karin and Lindahl, Anders and Skiöldebrand, Eva and Skioldebrand, Eva (2022). Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants. Equine Veterinary Journal
[Research article]

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Abstract

Background: Autologous conditioned serum (ACS) is used to treat osteoarthritis in horses, although its effects are not fully investigated.Objectives: To investigate the effects of equine serum and conditioned serum on chondrocytes stimulated with interleukin (IL)-1 beta and cartilage explants with mild osteoarthritis.Study design: In vitro experimental study.Methods: The effect of three different serum preparations (unincubated control [PS], serum incubated 24 h [PS24h] and serum incubated 24 h in ACS containers [PCS]) pooled from lame horses were tested in two in vitro models. IL-1 beta and IL-1 receptor antagonist (IL-1Ra) concentrations were measured in all sera. In model 1, chondrocyte pellet cultures were stimulated with IL-1 beta prior to treatment with the serum preparations for 2 and 48 h. Microarray, polymerase chain reaction, and matrix metallopeptidase-13 analyses were performed. In model 2, cartilage explants from horses with structural osteoarthritis were treated with PS or PCS on days 0, 6 and 12, or left untreated, and evaluated at day 24 using the OARSI grading scale for histological evaluation of articular cartilage.Results: The IL-1Ra concentration in PS24h and PCS was significantly higher than in PS. In model 1, inflammation- and cartilage matrix degradation-related genes were upregulated after 48 h in all treatment groups versus untreated controls. Cartilage matrix molecules, aggrecan and collagens, were downregulated in PS24h- and PCS-treated pellets versus untreated controls. Growth factor signalling genes were upregulated-FGF7 in all treatment groups, BMP2 in PS24h-, and INHBA in PCS-treated-compared with untreated controls. In model 2, the OARSI score at day 24 was not significantly different between treatment groups.Main limitations: Results from in vitro models cannot be directly translated to in vivo situations.Conclusions: In vitro treatment with conditioned serum did not alleviate IL-1 beta-induced responses in chondrocyte pellets or lead to morphological improvement in osteoarthritic cartilage explants.

Authors/Creators:Löfgren, Maria and Ekman, Stina and Ekholm, Josefine and Engstrom, Mona and Fjordbakk, Cathrine T. and Svala, Emilia and Holm Forsström, Karin and Lindahl, Anders and Skiöldebrand, Eva and Skioldebrand, Eva
Title:Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants
Series Name/Journal:Equine Veterinary Journal
Year of publishing :2022
Number of Pages:11
Publisher:WILEY
ISSN:0425-1644
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:autologous conditioned serum, horse, IL-1 receptor antagonist, inflammation, interleukin 1 beta, osteoarthritis
URN:NBN:urn:nbn:se:slu:epsilon-p-117555
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117555
Additional ID:
Type of IDID
DOI10.1111/evj.13582
Web of Science (WoS)000807250900001
ID Code:28569
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
University Animal Hospital
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
University Animal Hospital (UAH)
Deposited By: SLUpub Connector
Deposited On:26 Aug 2022 07:25
Metadata Last Modified:26 Aug 2022 07:31

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