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Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana

Zhang, Ai and Matsuoka, Keita and Valiya Kadavu, Abdul Kareem and Robert, Madalen and Roszak, Pawel and Blob, Bernhard and Bisht, Anchal and De Veylder, Lieven and Voiniciuc, Catalin and Asahina, Masashi and Melnyk, Charles (2022). Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana. Current Biology. 32 :9 , 1883-1893
[Research article]

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Abstract

Wound healing is a fundamental property of plants and animals that requires recognition of cellular damage to initiate regeneration. In plants, wounding activates a defense response via the production of jasmonic acid and a regeneration response via the hormone auxin and several ethylene response factor (ERF) and NAC domain-containing protein (ANAC) transcription factors. To better understand how plants recognize damage and initiate healing, we searched for factors upregulated during the horticulturally relevant process of plant grafting and found four related DNA binding with one finger (DOF) transcription factors, HIGH CAMBIAL ACTIVITY2 (HCA2), TARGET OF MONOPTEROS6 (TMO6), DOF2.1, and DOF6, whose expression rapidly activated at the Arabidopsis graft junction. Grafting or wounding a quadruple hca2, tmo6, dof2.1, dof6 mutant inhibited vascular and cell-wall-related gene expression. Furthermore, the quadruple dof mutant reduced callus formation, tissue attachment, vascular regeneration, and pectin methylesterification in response to wounding. We also found that activation of DOF gene expression after wounding required auxin, but hormone treatment alone was insufficient for their induction. However, modifying cell walls by enzymatic digestion of cellulose or pectin greatly enhanced TMO6 and HCA2 expression, whereas genetic modifications to the pectin or cellulose matrix using the PECTIN METHYLESTERASE INHIBITOR5 overexpression line or korrigan1 mutant altered TMO6 and HCA2 expression. Changes to the cellulose or pectin matrix were also sufficient to activate the wound-associated ERF115 and ANAC096 transcription factors, suggesting that cell-wall damage represents a common mechanism for wound perception and the promotion of tissue regeneration.

Authors/Creators:Zhang, Ai and Matsuoka, Keita and Valiya Kadavu, Abdul Kareem and Robert, Madalen and Roszak, Pawel and Blob, Bernhard and Bisht, Anchal and De Veylder, Lieven and Voiniciuc, Catalin and Asahina, Masashi and Melnyk, Charles
Title:Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana
Series Name/Journal:Current Biology
Year of publishing :2022
Volume:32
Number:9
Page range:1883-1893
Number of Pages:11
Publisher:CELL PRESS
ISSN:0960-9822
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Cell and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-117573
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-117573
Additional ID:
Type of IDID
DOI10.1016/j.cub.2022.02.069
Web of Science (WoS)000799211900016
ID Code:28648
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:30 Aug 2022 12:31
Metadata Last Modified:30 Aug 2022 12:41

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