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Aedes albopictus salivary proteins adenosine deaminase and 34k2 interact with human mast cell specific proteases tryptase and chymase

Li, Zhiqiang and Ji, Cejuan and Cheng, Jinzhi and Åbrink, Magnus and Shen, Tao and Kuang, Xiaoyuan and Shang, Zhengling and Wu, Jiahong (2022). Aedes albopictus salivary proteins adenosine deaminase and 34k2 interact with human mast cell specific proteases tryptase and chymase. Bioengineered. 13 :5 , 13752-13766
[Research article]

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Abstract

When mosquitoes probe to feed blood, they inoculate a mixture of salivary molecules into vertebrate hosts' skin causing acute inflammatory reactions where mast cell-derived mediators are involved. Mosquito saliva contains many proteins with largely unknown biological functions. Here, two Aedes albopictus salivary proteins - adenosine deaminase (alADA) and al34k2 - were investigated for their immunological impact on mast cells and two mast cell-specific proteases, the tryptase and the chymase. Mouse bone marrow-derived mast cells were challenged with increased concentrations of recombinant alADA or al34k2 for 1, 3, and 6 h, and to measure mast cell activation, the activity levels of beta-hexosaminidase and tryptase and secretion of IL-6 were evaluated. In addition, a direct interaction between alADA or al34k2 with tryptase or chymase was investigated. Results show that bone marrow-derived mast cells challenged with 10 mu g/ml of alADA secreted significant levels of beta-hexosaminidase, tryptase, and IL-6. Furthermore, both al34k2 and alADA are cut by human tryptase and chymase. Interestingly, al34k2 dose-dependently enhance enzymatic activity of both tryptase and chymase. In contrast, while alADA enhances the enzymatic activity of tryptase, chymase activity was inhibited. Our finding suggests that alADA and al34k2 via interaction with mast cell-specific proteases tryptase and chymase modulate mast cell-driven immune response in the local skin microenvironment. alADA- and al34k2-mediated modulation of tryptase and chymase may also recruit more inflammatory cells and induce vascular leakage, which may contribute to the inflammatory responses at the mosquito bite site.

Authors/Creators:Li, Zhiqiang and Ji, Cejuan and Cheng, Jinzhi and Åbrink, Magnus and Shen, Tao and Kuang, Xiaoyuan and Shang, Zhengling and Wu, Jiahong
Title:Aedes albopictus salivary proteins adenosine deaminase and 34k2 interact with human mast cell specific proteases tryptase and chymase
Series Name/Journal:Bioengineered
Year of publishing :2022
Volume:13
Number:5
Page range:13752-13766
Number of Pages:15
Publisher:TAYLOR and FRANCIS INC
ISSN:2165-5979
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Aedes albopictus, adenosine deaminase, 34k2, mast cell, tryptase, chymase
URN:NBN:urn:nbn:se:slu:epsilon-p-118295
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118295
Additional ID:
Type of IDID
DOI10.1080/21655979.2022.2081652
Web of Science (WoS)000816742600001
ID Code:28713
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:01 Sep 2022 11:56
Metadata Last Modified:01 Sep 2022 12:01

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