Home About Browse Search
Svenska


Genetic and Molecular Characterization of a New EMS-Induced Mutant without the Third Glucose Moiety at the C-3 Sugar Chain of Saponin in Glycine max (L.) Merr.

Ko, Jeongyun and Sundaramoorthy, Jagadeesh and Tsukamoto, Chigen and Park, Junbeom and Lee, Jinwon and Jo, Hyun and Lee, Jeong-Dong and Seo, Hak Soo and Song, Jong Tae (2022). Genetic and Molecular Characterization of a New EMS-Induced Mutant without the Third Glucose Moiety at the C-3 Sugar Chain of Saponin in Glycine max (L.) Merr. Agronomy. 12 :7 , 1598
[Research article]

[img] PDF
1MB

Abstract

Saponin, a secondary metabolite, is produced by various plant species, including soybean (Glycine max (L.) Merr.). Soybeans synthesize triterpenoid saponins, which are classified by their aglycone structure and sugar chain composition. Here, we characterized an ethyl methanesulfonate-induced mutant, PE1539, without saponin and with a glucose moiety at the third position of the C-3 sugar chain. The saponin phenotype of PE1539 is described by the accumulation of Ab-gamma g saponin and deficiency of Ab-alpha g saponin and DDMP-alpha g saponin, similar to a previously reported sg-3 mutant in soybean. Genetic analysis showed that the saponin phenotype of PE1539 is controlled by a recessive mutation. We mapped the gene responsible for the phenotype of PE1539 and the mapped region included Sg-3 (Glyma.10G104700). Further analysis of Sg-3 in PE1539 using DNA sequencing revealed a single-nucleotide substitution in the exon (G804A), resulting in a premature stop codon; thus, PE1539 produced a PSPG box-truncated protein. Saponin phenotype analysis of the F-2 population-from a cross between wild-type Uram and PE1539-showed that the phenotype of saponin was cosegregated with the genotype of Sg 3. Quantitative real-time PCR showed reduced expression of Sg-3 in PE1539 cells. Together, our data indicate that the saponin phenotype of PE1539 results from a mutation in Sg-3.

Authors/Creators:Ko, Jeongyun and Sundaramoorthy, Jagadeesh and Tsukamoto, Chigen and Park, Junbeom and Lee, Jinwon and Jo, Hyun and Lee, Jeong-Dong and Seo, Hak Soo and Song, Jong Tae
Title:Genetic and Molecular Characterization of a New EMS-Induced Mutant without the Third Glucose Moiety at the C-3 Sugar Chain of Saponin in Glycine max (L.) Merr.
Series Name/Journal:Agronomy
Year of publishing :2022
Volume:12
Number:7
Article number:1598
Number of Pages:11
Publisher:MDPI
ISSN:2073-4395
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:soybean, saponin, saponin phenotype, Sg-3, UDP-glycosyltransferase
URN:NBN:urn:nbn:se:slu:epsilon-p-118597
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118597
Additional ID:
Type of IDID
DOI10.3390/agronomy12071598
Web of Science (WoS)000831404400001
ID Code:28736
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:02 Sep 2022 07:25
Metadata Last Modified:02 Sep 2022 07:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits