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Usage of FTA (R) Classic Cards for Safe Storage, Shipment, and Detection of Arboviruses

Krambrich, Janina and Bringeland, Emelie and Hesson, Jenny C. and Hoffman, Tove and Lundkvist, Ake and Lindahl, Johanna F. and Lindahl, Johanna and Ling, Jiaxin (2022). Usage of FTA (R) Classic Cards for Safe Storage, Shipment, and Detection of Arboviruses. Microorganisms. 10 :7 , 1445
[Research article]

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Abstract

Infections caused by arthropod-borne RNA viruses are overrepresented among emerging infectious diseases. Effective methods for collecting, storing, and transporting clinical or biological specimens are needed worldwide for disease surveillance. However, many tropical regions where these diseases are endemic lack analytical facilities and possibility of continuous cold chains, which presents challenges from both a biosafety and material preservation perspective. Whatman (R) FTA (R) Classic Cards may serve as an effective and safe option for transporting hazardous samples at room temperature, particularly for RNA viruses classified as biosafety level (BSL) 2 and 3 pathogens, from sampling sites to laboratories. In this study, we investigated the biosafety and perseverance of representative alpha-and flaviviruses stored on FTA (R) cards. To evaluate the virus inactivation capacity of FTA (R) cards, we used Sindbis virus (SINV), chikungunya virus (CHIKV), and Japanese encephalitis virus (JEV). We inoculated susceptible cells with dilution series of eluates from viral samples stored on the FTA (R) cards and observed for cytopathic effect to evaluate the ability of the cards to inactivate viruses. All tested viruses were inactivated after storage on FTA (R) cards. In addition, we quantified viral RNA of JEV, SINV, and tick-borne encephalitis virus (TBEV) stored on FTA (R) cards at 4 degrees C, 25 degrees C, and 37 degrees C for 30 days using two reverse transcriptase quantitative PCR assays. Viral RNA of SINV stored on FTA (R) cards was not reduced at either 4 degrees C or 25 degrees C over a 30-day period, but degraded rapidly at 37 degrees C. For JEV and TBEV, degradation was observed at all temperatures, with the most rapid degradation occurring at 37 degrees C. Therefore, the use of FTA (R) cards provides a safe and effective workflow for the collection, storage, and analysis of BSL 2- and 3-virus RNA samples, but there is a risk of false negative results if the cards are stored at higher temperatures for long periods of time. Conscious usage of the cards can be useful in disease surveillance and research, especially in tropical areas where transportation and cold chains are problematic.

Authors/Creators:Krambrich, Janina and Bringeland, Emelie and Hesson, Jenny C. and Hoffman, Tove and Lundkvist, Ake and Lindahl, Johanna F. and Lindahl, Johanna and Ling, Jiaxin
Title:Usage of FTA (R) Classic Cards for Safe Storage, Shipment, and Detection of Arboviruses
Series Name/Journal:Microorganisms
Year of publishing :2022
Volume:10
Number:7
Article number:1445
Number of Pages:13
Publisher:MDPI
ISSN:2076-2607
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Infectious Medicine
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:biosafety, flavivirus, alphavirus, arboviruses, FTA (R) Classic Card, RNA stability
URN:NBN:urn:nbn:se:slu:epsilon-p-118598
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118598
Additional ID:
Type of IDID
DOI10.3390/microorganisms10071445
Web of Science (WoS)000832061800001
ID Code:28743
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:02 Sep 2022 08:32
Metadata Last Modified:02 Sep 2022 08:41

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