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Variation among stallions in sperm quality after single layer centrifugation

Al-Kass, Ziyad and Brown, Alexander and Johannisson, Anders and Ntallaris, Theodoros and Morrell, Jane (2021). Variation among stallions in sperm quality after single layer centrifugation. Reproduction in Domestic Animals. 56 :6 , 848-856
[Research article]

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Abstract

Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective of this study was to determine the variation among stallions in the effects of SLC on sperm quality during cooled storage for up to 48 hr. Semen samples from seven stallions (18 ejaculates) were split, with one portion being used for SLC and the other serving as a control (CON). Sperm quality (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) and chromatin integrity) were analysed at 0, 24 and 48 hr using computer-assisted sperm analysis and flow cytometry. Sperm quality was better in SLC than in CON at all timepoints, especially chromatin integrity and MI (p < .0001 for both), and some categories of ROS production (e.g. proportion of live hydrogen peroxide negative spermatozoa, p < .0001), but the degree of improvement varied among stallions and type of ROS (p p < .0001). Total and progressive motility were also better in SLC samples than in CON at 24 and 48 hr (p < .0001), although the effect on sperm kinematics varied. The interaction of treatment, time and stallion was not significant. In conclusion, sperm quality was better in SLC samples than in CON, although there was considerable individual variation among stallions. The improvement in sperm quality, particularly in chromatin integrity, was clearly beneficial, and therefore the use of this technique would be warranted for all stallion semen samples.

Authors/Creators:Al-Kass, Ziyad and Brown, Alexander and Johannisson, Anders and Ntallaris, Theodoros and Morrell, Jane
Title:Variation among stallions in sperm quality after single layer centrifugation
Series Name/Journal:Reproduction in Domestic Animals
Year of publishing :2021
Volume:56
Number:6
Page range:848-856
Number of Pages:9
ISSN:0936-6768
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:DNA fragmentation, equine semen, liquid stallion semen, semen evaluation, stored stallion sperm samples
URN:NBN:urn:nbn:se:slu:epsilon-p-111171
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111171
Additional ID:
Type of IDID
DOI10.1111/rda.13924
Web of Science (WoS)000630901600001
Scopus2-s2.0-85102731227
ID Code:28772
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:05 Sep 2022 09:25
Metadata Last Modified:05 Sep 2022 09:31

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