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Assembly of root-associated N₂O-reducing communities of annual crops is governed by selection for nosZ Glade I over Glade II

Graf, Daniel and Jones, Christopher and Zhao, Enid Ming and Hallin, Sara (2022). Assembly of root-associated N₂O-reducing communities of annual crops is governed by selection for nosZ Glade I over Glade II. FEMS Microbiology Ecology. 98 :9
[Research article]

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Abstract

The rhizosphere is a hotspot for denitrification. The nitrous oxide (N₂O) reductase among denitrifiers and nondenitrifying N₂O reducers is the only known N₂O sink in the biosphere. We hypothesized that the composition of root-associated N₂O-reducing communities when establishing on annual crops depend on soil type and plant species, but that assembly processes are independent of these factors and differ between nosZ clades I and II. Using a pot experiment with barley and sunflower and two soils, we analyzed the abundance, composition, and diversity of soil and root-associated N₂O reducing communities by qPCR and amplicon sequencing of nosZ. Clade I was more abundant on roots compared to soil, while clade II showed the opposite. In barley, this pattern coincided with N₂O availability, determined as potential N₂O production rates, but for sunflower no N₂O production was detected in the root compartment. Root and soil nosZ communities differed in composition and phylogeny-based community analyses indicated that assembly of root-associated N₂O reducers was driven by the interaction between plant and soil type, with inferred competition being more influential than habitat selection. Selection between clades I and II in the root/soil interface is suggested, which may have functional consequences since most clade I microorganisms can produce N₂O.

Authors/Creators:Graf, Daniel and Jones, Christopher and Zhao, Enid Ming and Hallin, Sara
Title:Assembly of root-associated N₂O-reducing communities of annual crops is governed by selection for nosZ Glade I over Glade II
Series Name/Journal:FEMS Microbiology Ecology
Year of publishing :2022
Volume:98
Number:9
Number of Pages:11
Publisher:OXFORD UNIV PRESS
ISSN:0168-6496
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:denitrification, nitrous oxide, rhizosphere, soil
URN:NBN:urn:nbn:se:slu:epsilon-p-118790
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118790
Additional ID:
Type of IDID
DOI10.1093/femsec/fiac092
Web of Science (WoS)000843705200004
ID Code:28780
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:05 Sep 2022 12:56
Metadata Last Modified:06 Sep 2022 07:58

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