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Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants

Murray, Kathryn A. and Kinney, Nina L. H. and Griffiths, Christopher and Hasan, Muhammad and Gibson, Matthew and Whale, Thomas F. (2022). Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants. Scientific Reports. 12 :1 , 12295
[Research article]

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Abstract

Cryopreservation of biological material is vital for existing and emerging biomedical and biotechnological research and related applications, but there remain significant challenges. Cryopreservation of cells in sub-milliliter volumes is difficult because they tend to deeply supercool, favoring lethal intracellular ice formation. Some tree pollens are known to produce polysaccharides capable of nucleating ice at warm sub-zero temperatures. Here we demonstrated that aqueous extractions from European hornbeam pollen (pollen washing water, PWW) increased ice nucleation temperatures in 96-well plates from approximate to - 13 degrees C to approximate to - 7 degrees C. Application of PWW to the cryopreservation of immortalized T-cells in 96-well plates resulted in an increase of post-thaw metabolic activity from 63.9% (95% CI [58.5 to 69.2%]) to 97.4% (95% CI [86.5 to 108.2%]) of unfrozen control. When applied to cryopreservation of immortalized lung carcinoma monolayers, PWW dramatically increased post-thaw metabolic activity, from 1.6% (95% CI [- 6.6 to 9.79%]) to 55.0% (95% CI [41.6 to 68.4%]). In contrast to other ice nucleating agents, PWW is soluble, sterile and has low cytotoxicity meaning it can be readily incorporated into existing cryopreservation procedures. As such, it can be regarded as a unique class of cryoprotectant which acts by inducing ice nucleation at warm temperatures.

Authors/Creators:Murray, Kathryn A. and Kinney, Nina L. H. and Griffiths, Christopher and Hasan, Muhammad and Gibson, Matthew and Whale, Thomas F.
Title:Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants
Series Name/Journal:Scientific Reports
Year of publishing :2022
Volume:12
Number:1
Article number:12295
Number of Pages:11
Publisher:NATURE PORTFOLIO
ISSN:2045-2322
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-118615
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118615
Additional ID:
Type of IDID
DOI10.1038/s41598-022-15545-4
Web of Science (WoS)000827810800064
ID Code:28783
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Aquatic Resources
Deposited By: SLUpub Connector
Deposited On:05 Sep 2022 14:57
Metadata Last Modified:05 Sep 2022 15:01

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