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Subclinical endometritis differentially affects the transcriptomic profiles of endometrial glandular, luminal, and stromal cells of postpartum dairy cows

Perelra, Goncalo and Guo, Yongzhi and Silva, Elisabete and Silva, Marta Filipa and Bevilacqua, Claudia and Charpigny, Gilles and Lopes-da-Costa, Luis and Humblot, Patrice (2022). Subclinical endometritis differentially affects the transcriptomic profiles of endometrial glandular, luminal, and stromal cells of postpartum dairy cows. Journal of Dairy Science. 105 :7 , 6125-6143
[Research article]

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Abstract

In postpartum dairy cows, subclinical endometritis flammation, which has profound detrimental effects on subsequent reproductive performance. To date, transcriptomic studies related to this condition were either based on biopsy-derived whole-endometrium tissue or endometrial swab or cytobrush samples, thus masking effects of disease on cell type-specific gene expression. This study tested the hypothesis that different endometrial health statuses are associated with distinct transcription profiles of endometrial stromal, glandular, and luminal epithelial cells. At 44 d postpartum (DPP), endometrial biopsies were taken from dairy or affected by persistent SCE, according to endometrial cytology taken at 21 and 44 DPP. Stromal, glandular, and luminal epithelial cells were isolated from the whole-tissue biopsy by laser capture microdissection, and the cell-specific transcription profiles were determined by RNA sequencing. Differential gene expression demonstrated that global transcriptomic profiles and corresponding lists of differentially expressed genes beamong cell types. Results also showed that although healthy and recovered cows presented similar endoprior presence of immune cells still affected the transcriptome of endometrial cells at this stage, delaying complete functional recovery. Recovery or persistence of inflammation was associated with gene expression patterns involved not only in immune function but also in tissue remodeling, cell adhesion, and uterine recep-tivity in a cell type-specific manner. Identifying these signatures may contribute to the development of novel diagnostic tools and therapeutic strategies. In addition, these results may help to define preventive measures or ways to stimulate recovery from endometrial inflamma-tion, thus helping to restore the fertility of postpartum dairy cows.

Authors/Creators:Perelra, Goncalo and Guo, Yongzhi and Silva, Elisabete and Silva, Marta Filipa and Bevilacqua, Claudia and Charpigny, Gilles and Lopes-da-Costa, Luis and Humblot, Patrice
Title:Subclinical endometritis differentially affects the transcriptomic profiles of endometrial glandular, luminal, and stromal cells of postpartum dairy cows
Series Name/Journal:Journal of Dairy Science
Year of publishing :2022
Volume:105
Number:7
Page range:6125-6143
Number of Pages:19
Publisher:ELSEVIER SCIENCE INC
ISSN:0022-0302
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:endometrium, subclinical endometritis, laser capture microdissection, transcriptome
URN:NBN:urn:nbn:se:slu:epsilon-p-118623
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118623
Additional ID:
Type of IDID
DOI10.3168/jds.2022-21811
Web of Science (WoS)000827977600013
ID Code:28799
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:07 Sep 2022 08:31
Metadata Last Modified:07 Sep 2022 08:41

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