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Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

Antoniadi, Ioanna and Skalicky, Vladimir and Sun, Guiling and Ma, Wen and Galbraith, David W. and Novak, Ondrej and Ljung, Karin (2022). Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis. Cytometry Part A. 101 :9 , 725-736
[Research article]

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Abstract

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.

Authors/Creators:Antoniadi, Ioanna and Skalicky, Vladimir and Sun, Guiling and Ma, Wen and Galbraith, David W. and Novak, Ondrej and Ljung, Karin
Title:Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis
Series Name/Journal:Cytometry Part A
Year of publishing :2022
Volume:101
Number:9
Page range:725-736
Number of Pages:12
Publisher:WILEY
ISSN:1552-4922
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Keywords:autofluorescence, best practices, plant flow cytometry and sorting, protoplasts, viability and integrity
URN:NBN:urn:nbn:se:slu:epsilon-p-112097
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112097
Additional ID:
Type of IDID
DOI10.1002/cyto.a.24461
Web of Science (WoS)000653233000001
Scopus2-s2.0-85106344118
ID Code:28828
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:12 Sep 2022 12:34
Metadata Last Modified:12 Sep 2022 13:19

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