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Effect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in rats

Mhd Omar, Nor Adila and Dicksved, Johan and Kruger, Johanita and Zamaratskaia, Galia and Michaelsson, Karl and Wolk, Alicja and Frank, Jan and Landberg, Rikard (2022). Effect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in rats. . 9 , 922336
[Research article]

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Abstract

Recent studies suggest that a diet rich in sugars significantly affects the gut microbiota. Adverse metabolic effects of sugars may partly be mediated by alterations of gut microbiota and gut health parameters, but experimental evidence is lacking. Therefore, we investigated the effects of high intake of fructose or galactose, with/without fructooligosaccharides (FOS), on gut microbiota composition in rats and explored the association between gut microbiota and low-grade systemic inflammation. Sprague-Dawley rats (n = 6/group) were fed the following isocaloric diets for 12 weeks (% of the dry weight of the sugars or FOS): (1) starch (control), (2) fructose (50%), (3) galactose (50%), (4) starch+FOS (15%) (FOS control), (5) fructose (50%)+FOS (15%), (6) galactose (50%)+FOS (15%), and (7) starch+olive (negative control). Microbiota composition in the large intestinal content was determined by sequencing amplicons from the 16S rRNA gene; 341F and 805R primers were used to generate amplicons from the V3 and V4 regions. Actinobacteria, Verrucomicrobia, Tenericutes, and Cyanobacteria composition differed between diets. Bifidobacterium was significantly higher in all diet groups where FOS was included. Modest associations between gut microbiota and metabolic factors as well as with gut permeability markers were observed, but no associations between gut microbiota and inflammation markers were observed. We found no coherent effect of galactose or fructose on gut microbiota composition. Added FOS increased Bifidobacterium but did not mitigate potential adverse metabolic effects induced by the sugars. However, gut microbiota composition was associated with several metabolic factors and gut permeability markers which warrant further investigations.

Authors/Creators:Mhd Omar, Nor Adila and Dicksved, Johan and Kruger, Johanita and Zamaratskaia, Galia and Michaelsson, Karl and Wolk, Alicja and Frank, Jan and Landberg, Rikard
Title:Effect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in rats
Year of publishing :2022
Volume:9
Article number:922336
Number of Pages:15
Publisher:FRONTIERS MEDIA SA
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:fructose, galactose, fructooligosaccharides, gut microbiota, 16S rRNA
URN:NBN:urn:nbn:se:slu:epsilon-p-118854
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118854
Additional ID:
Type of IDID
DOI10.3389/fnut.2022.922336
Web of Science (WoS)000844961200001
ID Code:28838
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(VH) > Dept. of Animal Nutrition and Management
(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:13 Sep 2022 14:25
Metadata Last Modified:13 Sep 2022 14:31

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