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Determination of conifer age biomarker DAL1 interactome using Y2H-seq

Chen, Xi and Zhu, Qianya and Nie, Yumeng and Han, Fangxu and Li, Yue and Wu, Harry and Niu, Shihui (2021). Determination of conifer age biomarker DAL1 interactome using Y2H-seq. Forestry Research. 1 , 12
[Research article]

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Abstract

Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.

Authors/Creators:Chen, Xi and Zhu, Qianya and Nie, Yumeng and Han, Fangxu and Li, Yue and Wu, Harry and Niu, Shihui
Title:Determination of conifer age biomarker DAL1 interactome using Y2H-seq
Series Name/Journal:Forestry Research
Year of publishing :2021
Volume:1
Article number:12
Number of Pages:12
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203)
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Medical Biotechnology
Keywords:Ageing, Conifer, DAL1, Proteins-interaction, Y2H-seq
URN:NBN:urn:nbn:se:slu:epsilon-p-118407
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-118407
Additional ID:
Type of IDID
DOI10.48130/FR-2021-0012
ID Code:28849
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:15 Sep 2022 09:03
Metadata Last Modified:15 Sep 2022 09:11

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