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The pathogenesis of the porcine rubulavirus (LPMV)

infection in PK-15 cultured cells and in experimentally infected pigs

Hernández-Jáuregui y Alvarez, Pablo (2003). The pathogenesis of the porcine rubulavirus (LPMV). Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Veterinaria, 1401-6257 ; 155
ISBN 91-576-6381-5
[Doctoral thesis]

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Abstract

This thesis summarises and discusses the results of in vitro and in vivo studies on the pathogenesis of porcine rubulavirus infection. Study I describes the development of a blocking ELISA for screening antibodies against PoRV-LPMV. This ELISA is based on a monoclonal antibody (Mab) against the haemagglutinin (HN) protein and has a sensitivity of 99% and specificity of 97%. Study II describes the internalisation of the virus into PK-15 cells using immuno-gold labelled Mabs. The HN protein was detected at the surface and in the cytoplasm of the cells as small and large aggregates after 10 min and 24 h post-infection (pi), respectively. The nucleoprotein (NP) was detected after 24 h pi. Study III describes apoptotic cells in lymphoid tissue and changes in lymphocyte subpopulations in piglets infected with PoRV-LPMV at 5 or 17 days of age. All piglets had increased numbers of apoptotic cells, and the proportions of CD2+, CD8, CD4+ and MHC class II T cells were reduced in piglets infected at 5 days. MHC class II cells remained elevated for 15 days in piglets infected at 17 days. Study IV describes the pathogenesis of central nervous and respiratory system lesions in piglets infected with PoRV-LPMV at 3 and 17 days of age. The younger pigs were more susceptible to infection and developed interstitial pneumonia and encephalomyelitis. Similar but essentially similar lesions were found in the older pigs. Study V describes epididymal lesions in sexually mature boars infected with PoRV-LPMV. These animals developed epididymal necrosis, sperm granulomas and atrophy of the testis. Study VI is an analysis of the effects of the virus in pregnant gilts. Mummies and foetuses with dermal haemorrhages were found and PoRV-LPMV was isolated from foetal brain and lung tissues. The gilts remained clinically normal although virus was isolated from the lung, ovaries and placenta.

Authors/Creators:Hernández-Jáuregui y Alvarez, Pablo
Title:The pathogenesis of the porcine rubulavirus (LPMV)
Subtitle:infection in PK-15 cultured cells and in experimentally infected pigs
Series/Journal:Acta Universitatis agriculturae Sueciae. Veterinaria (1401-6257)
Year of publishing :June 2003
Volume:155
Number of Pages:29
Papers/manuscripts:
NumberReferences
ALLI. A. Nordengrahn, M. Svenda. J. Moreno-López, A. Bergvall, P. Hernández, F. McNeilly, G. Allan. M.Merza. Development of a blocking ELISA for screening antibodies to porcine rubulavirus, La Piedad Michoacan Virus. J.Vet. Diagn Invest. (1999) 11: 319-323. II. Pablo Hernández Jáuregui, Alia Jacoub, Seamus Kennedy, Bill Curran, Carlos Téllez, Martín Svenda, Leif Ljung, Jorge Moreno-López. The uptake of the porcine rubulavirus LPMV (La Piedad Michoacán Virus) in PK-15 cells. Arch. Med. Res. (2001) 32: 400-409. III. A. Rodríguez Ropón, P. Hernández Jáuregui, L. Sánchez-Torres, L. Favila-Castillo, S. Estrada Parra, J. Moreno-López, S. Kennedy. Porcine rubulavirus causes apoptosis in lymph nodes and increases CD8+ peripheral lymphocytes in piglets. J. Comp. Path. (2003) 128: 1-8. IV. P. Hernández Jáuregui, B. Herron, D. Moffett, S. Black, S. McConnell, B. Adair, T. Linné, J. Moreno-López, S. Kennedy. Pathogenesis of Porcine rubulavirus Experimental infection in piglets. Manuscript. V. H. Ramírez Mendoza, P. Hernández Jáuregui, J. Reyes Leyva, E. Zenteno, J. Moreno-López, and S. Kennedy. Lesions in the reproductive tract of boars experimentally infected with porcine rubulavirus. J. Comp. Path. (1997): 117: 237-252. VI. P. Hernández Jáuregui, H. Ramírez Mendoza, C. Mercado García, J. Moreno-López and S. Kennedy. Experimental infection of pregnant gilts exposed to Porcine rubulavirus. J. Comp. Path. accepted (2003).
Place of Publication:Uppsala
Publisher:Dept. of Veterinary Microbiology, Swedish Univ. of Agricultural Sciences
ISBN for printed version:91-576-6381-5
ISSN:1401-6257
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:Not in use, please see Agris categories
Agrovoc terms:swine, paramyxoviridae, replication, central nervous system, apoptosis, in vitro experimentation
Keywords:pig paramyxovirus, virus replication, central nervous system, apoptosis, reproductive tract lesions
URN:NBN:urn:nbn:se:slu:epsilon-54
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-54
ID Code:302
Deposited By: Staff Epsilon
Deposited On:10 Jun 2003 00:00
Metadata Last Modified:17 Apr 2015 10:18

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