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Biosensor assay for determination of haptoglobin in bovine milk
Åkerstedt, Maria and Björk, Lennart and Persson Waller, Karin and Sternesjö, Åse
(2006).
Biosensor assay for determination of haptoglobin in bovine milk.
Journal of dairy research. 73
, 299-305
[Research article]
Official URL: http://journals.cambridge.org/action/displayJourna... AbstractDespite more than 30 years of research into mastitis diagnostics, there are few alternatives to the somatic cell count (SCC) in practical use for identification of cows with subclinical mastitis. Mastitis is not only an animal welfare problem, but also affects the yield, composition and technological properties of milk. Hence, dairy cooperatives give farmers a premium quality payment to encourage low SCC although there is no clear scientific data defining the level of SCC in bulk tank milk that is associated with additional benefits in terms of milk quality. Recent research on alternative markers for inflammatory reactions in the lactating cow, e.g. in mastitis, includes investigations of the acute phase protein, haptoglobin (Hp). So far, the content of Hp in milk has mainly been studied in relation to mastitis diagnostics, with little attention given to its importance for milk composition and technological properties. At present, Hp in milk is measured using ELISA, but this technique is not suitable for routine large-scale analysis. In recent years, optical biosensor technology has been used for automated and rapid quantitative analysis of different components in milk, but so far not for analysis of acute phase proteins. The aim of the present study was to develop a rapid and sensitive biosensor method to determine Hp in milk. An affinity sensor assay based on the interaction between Hp and haemoglobin was developed using surface plasmon resonance (SPR) biosensor technology. The assay was used to analyse Hp in composite milk samples from cows without any clinical signs of mastitis and quarter milk samples with a weak to strong reaction in the California Mastitis Test (CMT). A commercial ELISA for determination of Hp in milk was used for comparison. The limit of detection (LOD) of the biosensor assay was determined as 1.1 mg/l. Within-assay and betweenday variations were determined both with bulk tank milk spiked with human Hp and with composite milk samples containing bovine Hp. Coefficients of variation varied between 3.6 and 8.6% at concentrations between 4.0 and 12 mg/l, respectively. Agreement between the results obtained by the biosensor assay and the ELISA was satisfactory ; however, the results obtained by the biosensor were generally lower than the results obtained by the ELISA. Possible explanations for this observation are discussed. Authors/Creators: | Åkerstedt, Maria and Björk, Lennart and Persson Waller, Karin and Sternesjö, Åse |
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Title: | Biosensor assay for determination of haptoglobin in bovine milk |
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Series Name/Journal: | Journal of dairy research |
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Year of publishing : | 2006 |
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Volume: | 73 |
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Page range: | 299-305 |
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Publisher: | Cambridge University Press |
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ISSN: | 1469-7629 |
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Language: | English |
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Additional Information: | The original publication is available at www.cambridge.org |
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Publication Type: | Research article |
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Refereed: | Yes |
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Full Text Status: | Public |
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Subjects: | Obsolete subject words > VETERINARY MEDICINE > Pathology |
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Keywords: | Mastitis diagnostics, dairy cow |
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ID Code: | 3243 |
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Department: | (NL, NJ) > Dept. of Food Science (until 161231) (VH) > Dept. of Clinical Sciences |
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Deposited By: |
Staff Epsilon
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Deposited On: | 15 May 2008 00:00 |
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Metadata Last Modified: | 02 Dec 2014 10:20 |
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Repository Staff Only: item control page Related resources Related document* | Åkerstedt, Maria and Persson Waller, Karin and Sternesjö, Åse (2007). Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. Journal of dairy research. 74:3, 198-203 | Related document* | Åkerstedt, Maria and Persson Waller, Karin and Bach Larsen, Lotte and Forsbäck, Linda and Sternesjö, Åse (2008). Relationship between haptoglobin and serum amyloid A in milk and milk quality. International dairy journal. 18:6, 669-674 | Related document* | Åkerstedt, Maria and Persson Waller, Karin and Sternesjö, Åse (2009). Haptoglobin and serum amyloid A in bulk tank milk in relation to raw milk quality. Journal of dairy research. 76:4, 483-489 | Related document* | Åkerstedt, Maria (2008). Bovine acute phase proteins in milk. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:16 | Related document* | Bernes, Gun and Persson Waller, Karin (2006). Vitaminer. Umeå: Fakulteten för naturresurser och lantbruksvetenskap > Institutionen för norrländsk jordbruksvetenskap, Sveriges lantbruksuniversitet. Nytt från Institutionen för Norrländsk jordbruksvetenskap. | References* | Petersen, H.H., Nielsen, J.P., Heegaard, P.M.H. Application of acute phase protein measurements in veterinary clinical chemistry (2004) Veterinary Research, 35 (2), pp. 163-187 | References* | Murata, H., Shimada, N., Yoshioka, M. Current research on acute phase proteins in veterinary diagnosis: An overview (2004) Veterinary Journal, 168 (1), pp. 28-40. | References* | Gruys, E., Obwolo, M.J., Toussaint, M.J.M. Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry: A review (1994) Vet. Bull., 64 (11), pp. 1009-1018 | References* | Eckersall, P.D., Young, F.J., McComb, C., Hogarth, C.J., Safi, S., Weber, A., McDonald, T., Nolan, A.M., Fitzpatrick, J.L. Acute phase proteins in serum and milk from dairy cows with clinical mastitis (2001) Veterinary Record, 148 (2), pp. 35-41. | References* | Pyörälä, S. Indicators of inflammation in the diagnosis of mastitis (2003) Veterinary Research, 34 (5), pp. 565-578. Cited 114 times. | References* | Makimura, S., Suzuki, N. Quantitative determination of bovine serum Haptoglobin and its elevation in some inflammatory diseases. (1982) Nippon juigaku zasshi. The Japanese journal of veterinary science, 44 (1), pp. 15-21. | References* | Grönlund, U., Hultén, C., Eckersall, P.D., Hogarth, C., Waller, K.P. Haptoglobin and serum amyloid A in milk and serum during acute and chronic experimentally induced Staphylococcus aureus mastitis (2003) Journal of Dairy Research, 70 (4), pp. 379-386. | References* | E, C. Regulation No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin (2004) Official Journal of the European Union, L226, pp. 22-82. | References* | Eckersall, P.D. The time is right for acute phase protein assays (2004) Veterinary Journal, 168 (1), pp. 3-5. Cited 49 times. | References* | Grönlund, U., Hallén Sandgren, C., Persson Waller, K. Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis (2005) Veterinary Research, 36 (2), pp. 191-198. | Is referenced by* | Smolenski, G.A., Wieliczko, R.J., Pryor, S.M., Broadhurst, M.K., Wheeler, T.T., Haigh, B.J. The abundance of milk cathelicidin proteins during bovine mastitis (2011) Veterinary Immunology and Immunopathology, 143 (1-2), pp. 125-130. | Is referenced by* | Pyörälä, S., Hovinen, M., Simojoki, H., Fitzpatrick, J., Eckersall, P.D., Orro, T. Acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens (2011) Veterinary Record, 168 (20), p. 535. | Is referenced by* | Åkerstedt, M., Forsbck, L., Larsen, T., Svennersten-Sjaunja, K. Natural variation in biomarkers indicating mastitis in healthy cows (2011) Journal of Dairy Research, 78 (1), pp. 88-96. | Is referenced by* | Trevisi, E., Zecconi, A., Bertoni, G., Piccinini, R. Blood and milk immune and inflammatory profiles in periparturient dairy cows showing a different liver activity index (2010) Journal of Dairy Research, 77 (3), pp. 310-317. | Is referenced by* | Larsen, T., Rntved, C.M., Ingvartsen, K.L., Vels, L., Bjerring, M. Enzyme activity and acute phase proteins in milk utilized as indicators of acute clinical E. coli LPS-induced mastitis (2010) Animal, 4 (10), pp. 1672-1679. |
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