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Bestämning av förekomst av patogena svampar i vete med PCR-teknik

Almquist, Charlotta and Lerenius, Cecilia and Filipsson, Caroline and Jonsson, Anders (2008). Bestämning av förekomst av patogena svampar i vete med PCR-teknik. Technical Report. Skara: (NL, NJ) > Dept. of Soil Sciences > Div. of Precision Agriculture, Sveriges lantbruksuniversitet. Rapport (Avdelningen för precisionsodling, Sveriges lantbruksuniversitet) ; 18
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Abstract

Today, the quantification of leaf pathogens on wheat mainly relies on a visual inspection and estimation of the percentage of infected leaf area. However, this is both time consuming, requires skilled personnel with experience in plant pathology and may also be misleading if several pathogens are present on the same leaf or if the symptoms are concealed by abiotic factors. More reliable methods suitable for routine diagnosis are necessary to be able to better evaluate and optimise the use of fungicides and different cultivars. In the SLF-project V0648026 "PCR based detection of plant pathogens in wheat", quantitative real-time PCR assays were developed for detection of the three leaf pathogens in winter wheat; Septoria tritici, Drechslera tritici-repentis (DTR) and Stagonospora nodorum. The amount of plant pathogen DNA was determined using relative quantification and a wheat-specific gene was used as reference. With high specificity, high reproducibility and low detection limit, the PCR methods developed enable an objective measurement of fungal infection levels in wheat. The results show a good correlation between visual grading and relative qPCR measurements. Although the visual grading did not report the presence of S. nodorum, this fungi was frequently detected using the qPCR assay. The results also show that qPCR can be used to study the effect of fungicides on the fungal flora with very small variation between replications. In addition, it was shown that qPCR is a useful tool for quantifying the amount of the three wheat pathogens in field trials where different cultivars are evaluated. Pre-symptomatic detection of the pathogens was possible and results show that the PCR-techniques even could be used to evaluate the presence of a few single spores. For the future the results indicates a possibility to improve the evaluation of plant pathogens and plant production systems.

Authors/Creators:Almquist, Charlotta and Lerenius, Cecilia and Filipsson, Caroline and Jonsson, Anders
Title:Bestämning av förekomst av patogena svampar i vete med PCR-teknik
Series/Journal:Rapport (Avdelningen för precisionsodling, Sveriges lantbruksuniversitet) (1652-2788)
Year of publishing :2008
Number:18
Number of Pages:42
Place of Publication:Skara
Publisher:Avdelningen för precisionsodling, Institutionen för markvetenskap, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-85911-51-6
ISSN:1652-2788
Language:Swedish
Publication Type:Report
Refereed:No
Full Text Status:Public
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Plant production
Keywords:PCR-teknik, patogena svampar
URN:NBN:urn:nbn:se:slu:epsilon-1-70
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1-70
ID Code:3292
Department:(NL, NJ) > Dept. of Soil Sciences > Div. of Precision Agriculture
Deposited By: Anna Nyberg
Deposited On:11 Aug 2008 00:00
Metadata Last Modified:02 Dec 2014 10:20

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