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A conditional marker gene allowing both positive and negative selection in plants

Erikson, Oskar and Hertzberg, Magnus and Näsholm, Torgny (2004). A conditional marker gene allowing both positive and negative selection in plants. Nature. Biotechnology. 22:4, 455-458
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Official URL: http://dx.doi.org/10.1038/nbt946

Abstract

Selectable markers enable transgenic plants or cells to be identified after transformation. They can be divided into positive and negative markers conferring a selective advantage or disadvantage, respectively. We present a marker gene, dao1, encoding D-amino acid oxidase (DAAO, EC 1.4.3.3) that can be used for either positive or negative selection, depending on the substrate. DAAO catalyzes the oxidative deamination of a range of D-amino acids. Selection is based on differences in the toxicity of different D-amino acids and their metabolites to plants. Thus, D-alanine and D-serine are toxic to plants, but are metabolized by DAAO into nontoxic products, whereas D-isoleucine and D-valine have low toxicity, but are metabolized by DAAO into the toxic keto acids 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate, respectively. Hence, both positive and negative selection is possible with the same marker gene. The marker has been successfully established in Arabidopsis thaliana, and proven to be versatile, rapidly yielding unambiguous results, and allowing selection immediately after germination.

Authors/Creators:Erikson, Oskar and Hertzberg, Magnus and Näsholm, Torgny
Title:A conditional marker gene allowing both positive and negative selection in plants
Series/Journal:Nature. Biotechnology (1087-0156)
Year of publishing :2004
Volume:22
Number:4
Page range:455-458
Publisher:Nature Publishing Group
ISSN:1087-0156
Language:English
Additional Information:The original publication is available at Nature Biotechnology http://dx.doi.org/10.1038/nbt946
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING
Keywords:transgenic plants, cells, genes, selection, dao1, D-amino acid oxidase
URN:NBN:urn:nbn:se:slu:epsilon-1-75
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1-75
Additional ID:
Type of IDID
DOI10.1038/nbt946
ID Code:3383
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: Malin Persson
Deposited On:06 Nov 2008 00:00
Metadata Last Modified:02 Dec 2014 10:21

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