Erikson, Oskar and Hertzberg, Magnus and Näsholm, Torgny
(2004).
A conditional marker gene allowing both positive and negative selection in plants.
Nature. Biotechnology. 22
:4
, 455-458
[Research article]
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Official URL: http://dx.doi.org/10.1038/nbt946
Abstract
Selectable markers enable transgenic plants or cells to be identified after transformation. They can be divided into positive and negative markers conferring a selective advantage or disadvantage, respectively. We present a marker gene, dao1, encoding D-amino acid oxidase (DAAO, EC 1.4.3.3) that can be used for either positive or negative selection, depending on the substrate. DAAO catalyzes the oxidative deamination of a range of D-amino acids. Selection is based on differences in the toxicity of different D-amino acids and their metabolites to plants. Thus, D-alanine and D-serine are toxic to plants, but are metabolized by DAAO into nontoxic products, whereas D-isoleucine and D-valine have low toxicity, but are metabolized by DAAO into the toxic keto acids 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate, respectively. Hence, both positive and negative selection is possible with the same marker gene. The marker has been successfully established in Arabidopsis thaliana, and proven to be versatile, rapidly yielding unambiguous results, and allowing selection immediately after germination.
Authors/Creators: | Erikson, Oskar and Hertzberg, Magnus and Näsholm, Torgny | ||||
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Title: | A conditional marker gene allowing both positive and negative selection in plants | ||||
Series Name/Journal: | Nature. Biotechnology | ||||
Year of publishing : | 2004 | ||||
Volume: | 22 | ||||
Number: | 4 | ||||
Page range: | 455-458 | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1087-0156 | ||||
Language: | English | ||||
Additional Information: | The original publication is available at Nature Biotechnology http://dx.doi.org/10.1038/nbt946 | ||||
Publication Type: | Research article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Accepted version | ||||
Full Text Status: | Public | ||||
Subjects: | Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING | ||||
Keywords: | transgenic plants, cells, genes, selection, dao1, D-amino acid oxidase | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-1-75 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1-75 | ||||
Additional ID: |
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Alternative URL: | http://www.nature.com/nbt/journal/v22/n4/abs/nbt946.html | ||||
ID Code: | 3383 | ||||
Department: | (S) > Dept. of Forest Genetics and Plant Physiology | ||||
Deposited By: | Malin Persson | ||||
Deposited On: | 06 Nov 2008 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:21 |
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