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Reproductive physiology of the female cat

with special reference to cervical patency, sperm distribution and hysterography

Chatdarong, Kaywalee (2003). Reproductive physiology of the female cat. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Veterinaria, 1401-6257 ; 162
ISBN 91-576-6395-5
[Doctoral thesis]

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Abstract

Ovarian cyclicity induces considerable changes in morphology, physiology and function of the reproductive organs in animals. This thesis aimed to study reproductive physiology of the female domestic cat, focusing on the cervix, uterus and uterine tube during different stages of the oestrous cycle, after mating and under pathological conditions. Transcervical catheterisation was performed using a specially designed catheter. Patency of the cervix and uterine motility were studied with the aids of fluoroscopy and scintigraphy by depositing contrast fluids and radiopharmaceutical medium in the cranial vagina. The relationship between cervical patency and oestrous behaviour, cornification of the vaginal cells and serum concentrations of oestradiol-17β were evaluated. Hysterography, a positive contrast study, was performed to illustrate the uterine appearance during the oestrous cycle in relation to histological characteristics of the endometrium. Immunohistochemistry using proliferating nuclear antigen (PCNA) was introduced to identify mitotic activity of the endometrial cells. Patency of the cervix to spermatozoa as well as sperm distribution after natural mating was evaluated by flushing the vagina, the uterus and the uterine tube. For the study of sperm distribution in the female reproductive tract, two methods, flushing and tissue sectioning were evaluated and compared. Using a specially designed catheter it was possible to catheterise the cervix during interoestrus, oestrus, metoestrus and postpartum and to introduce contrast fluids into the uterus to study uterine appearance using hysterography also when the cervix was closed. The period when the cervix was patent was found to vary among individuals: the cervix was open either only during late-oestrus; during midoestrus and late-oestrus; or throughout the entire behavioural oestrus. Patency of the cervix was found to usually coincide with the maximum degree of vaginal cornification and thus, presumably is regulated at least in part by the serum concentration of oestradiol-17β. Hysterograms revealed differences in uterine luminal shape that corresponded to the histological characteristics of the endometrium in cats at various stages of the oestrous cycle, cats given medroxyprogesterone acetate (MPA) and cats with uterine pathology. Straight- and wavy-shaped uterine lumens were characteristic of the uterine horns in the inactive and follicular stages of the oestrous cycle, respectively. Coil-shaped uterine lumen appeared to be a progestagenic effect seen in the luteal, the MPA-treated and the pathological groups. A coiled uterine cavity was suggestive of endometrial hyperplasia, whereas irregular filling defects were indicative of generalised cystic changes in the endometrium. The expression of PCNA in luminal and glandular epithelial cells was observed although the mitotic activity was not related to neither stages of oestrous cycle nor uterine pathological conditions. The results from the sperm distribution study demonstrated that the cervix and the uterotubal junction (UTJ) were sperm barriers in the cat. The endometrial crypts and the UTJ functioned as sperm reservoirs before ovulation, whereas the isthmus was a sperm reservoir around the time of ovulation. The observations determined the dynamics of the cervix, the uterus and the uterine tube in the cat in relation to ovarian activity. This thesis is the first to provide nomenclatures for describing uterine appearance in hysterograms according to the shape of the uterine horns, luminal cavity and the characteristics of the intraluminal lining, to demonstrate the PCNA expression as well as to determine the distribution of spermatozoa in the female reproductive tract of the cat after natural mating.

Authors/Creators:Chatdarong, Kaywalee
Title:Reproductive physiology of the female cat
Subtitle:with special reference to cervical patency, sperm distribution and hysterography
Year of publishing :September 2003
Volume:162
Number of Pages:51
Papers/manuscripts:
NumberReferences
I.Chatdarong, K., Lohachit, C., Ponglowhapan, S. & Linde-Forsberg, C. 2001. Transcervical catheterization and cervical patency in domestic cats. Journal of Reproduction and Fertility Supplement 57, 353-356.
II.Chatdarong, K., Kampa, N., Axnér, E. & Linde-Forsberg, C. 2002. Investigation of cervical patency and uterine appearance in domestic cats by fluoroscopy and scintigraphy. Reproduction in Domestic Animals 37, 275-281.
III.Chatdarong, K., Rungsipipat, A., Axnér, E. & Linde-Forsberg, C. Hysterographic appearance and uterine histology at different stages of the reproductive cycle and after progestagen treatment in the domestic cat. Manuscript submitted for publication.
IV.Chatdarong, K., Lohachit, C. & Linde-Forsberg, C. Distribution of spermatozoa in the female reproductive tract of the domestic cat in relation to ovulation induced by natural mating. Manuscript submitted for publication.
Place of Publication:Uppsala
Publisher:Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare
ISBN for printed version:91-576-6395-5
ISSN:1401-6257
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L74 Miscellaneous animal disorders
L Animal production > L53 Animal physiology - Reproduction
Subjects:Not in use, please see Agris categories
Agrovoc terms:cats, females, copulation, oviducts, reproduction, radiography, uterus, hyperplasia
Keywords:feline, queen, copulation, oviduct, radiography, endometrial hyperplasia
URN:NBN:urn:nbn:se:slu:epsilon-90
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-90
ID Code:364
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:Other
Deposited By: Kaywalee Chatdarong
Deposited On:30 Sep 2003 00:00
Metadata Last Modified:02 Dec 2014 10:04

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