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Studies of molecular mechanisms integrating carbon metabolism and growth in plants

Thelander, Mattias (2003). Studies of molecular mechanisms integrating carbon metabolism and growth in plants. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Agraria, 1401-6249 ; 432
ISBN 91-576-6474-9
[Doctoral thesis]

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Abstract

Plants use light energy, carbon dioxide and water to produce sugars and other carbohydrates, which serve as stored energy reserves and as building blocks for biosynthetic reactions. Supply of light is variable and plants have evolved means to adjust their growth and development accordingly. An increasing body of evidence suggests that the basic mechanisms for sensing and signaling energy availability in eukaryotes are evolutionary conserved and thus shared between plants, animals and fungi. I have used different experimental approaches that take advantage of findings from other eukaryotes in studying carbon and energy metabolism in plants. In the first part, I developed a novel screening procedure in yeast aimed at isolating cDNAs from other organisms encoding proteins with a possible function in sugar sensing or signaling. The feasibility of the method was confirmed by the cloning of a cDNA from Arabidopsis thaliana encoding a new F-box protein named AtGrh1, which is related to the yeast Grr1 protein that is involved in glucose repression. In the second part of the study, plant homologues of key components in the yeast glucose repression pathway were cloned and characterized in the moss Physcomitrella patens, in which gene function can be studied by gene targeting. We first cloned PpHXK1 which was shown to encode a chloroplast localized hexokinase representing a previously overlooked class of plant hexokinases with an N-terminal chloroplast transit peptide. Significantly, PpHxk1 is the major hexokinase in Physcomitrella, accounting for 80% of the glucose phosphorylating activity. A knockout mutant deleted for PpHXK1 exhibits a complex phenotype affecting growth, development and sensitivities to plant hormones. I also cloned and characterized two closely related Physcomitrella genes, PpSNF1a and PpSNF1b, encoding type 1 Snf1-related kinases. A double knockout mutant for these genes was viable even though it lacks detectable Snf1-like kinase activity. The mutant suffers from pleiotropic phenotypes which may reflect a constitutive high energy growth mode. Significantly, the double mutant requires constant high light and is therefore unable to grow in a normal day/night light cycle. These findings are consistent with the proposed role of the Snf1-related kinases as energy gauges which are needed to recognize and respond to low energy conditions.

Authors/Creators:Thelander, Mattias
Title:Studies of molecular mechanisms integrating carbon metabolism and growth in plants
Year of publishing :November 2003
Volume:432
Number of Pages:43
Papers/manuscripts:
NumberReferences
ALLI. Thelander, M., Fredriksson, D., Schouten, J., Hoge, H. & Ronne, H. 2002. Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that turns on glucose repression. Plant Mol. Biol. 49, 67-79. II. Olsson, T., Thelander, M. & Ronne, H. 2003. A novel type of chloroplast stromal hexokinase is the major glucose phosphorylating enzyme in the moss Physcomitrella patens. J. Biol. Chem. 278, 44439-44447. III. Thelander, M., Olsson, T. & Ronne, H. 2003. Snf1-related protein kinase 1 is needed for growth in a normal day-night light cycle. (Manuscript) IV. Thelander, M., Olsson, T. & Ronne, H. 2003. Phenotypic characterization of protonemal growth and development in a Physcomitrella patens hexokinase knockout mutant. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:91-576-6474-9
ISSN:1401-6249
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F61 Plant physiology - Nutrition
Subjects:Not in use, please see Agris categories
Agrovoc terms:biosynthesis, carbon cycle, energy metabolism, cloning, plant physiology, genetic engineering
Keywords:AMPK, hexokinase, glucose repression, Grr1, Snf1, SnRK1
URN:NBN:urn:nbn:se:slu:epsilon-102
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-102
ID Code:394
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Mattias Thelander
Deposited On:12 Nov 2003 00:00
Metadata Last Modified:02 Dec 2014 10:04

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