Högberg, Karl-Anders
(2003).
Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae. Silvestria, 1401-6230
; 294
ISBN 91-576-6528-1
[Doctoral thesis]
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Abstract
The use of vegetative mass propagation in practical forestry with Norway spruce (Picea abies (L.) Karst.) is limited at present, although its potential to deliver high genetic gains is obvious. The objective of this thesis was to study possibilities and limitations of vegetative propagation when applied in different parts of a breeding/mass propagation system for Norway spruce. Two vegetative propagation methods were studied: somatic embryogenesis and cutting propagation. Somatic embryogenesis was accompanied by losses of genotypes during the propagation process. The embryogenic response at proliferation and maturation was under family control, while germination was obtained for all families. Parental effects on proliferation and maturation were found for male parents but not for female. However, no correlations between embryogenic characters and breeding goal traits could be detected on parental level. Shortening of treatment with abscisic acid (ABA) during somatic embryo development gave pronounced positive effects on height growth of regenerated plants. An improved protocol, including five weeks ABA treatment and root development in liquid medium significantly improved performance of the resulting plants. The number of plants with lateral roots at the time of ex vitro transfer increased substantially with this protocol. Lateral roots at ex vitro transfer were shown to be a marker for good height growth and clonal uniformity during the next two years. Selection for height of cutting propagated clones in the nursery resulted in low responses in height after six years in field. The likely reason for this was low correlations between nursery traits and field traits. Genotype x environment interactions in the studied clonal test series varied from close to zero to more than 50% of the clone component. A tendency towards increased interaction components with age was obtained in one of the series. In situations with large genotype x environment interactions, clonal stability over sites should be included in the selection criteria.
Authors/Creators: | Högberg, Karl-Anders | ||||
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Title: | Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce | ||||
Series Name/Journal: | Acta Universitatis Agriculturae Sueciae. Silvestria | ||||
Year of publishing : | November 2003 | ||||
Number: | 294 | ||||
Number of Pages: | 39 | ||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||
ISBN for printed version: | 91-576-6528-1 | ||||
ISSN: | 1401-6230 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agris subject categories.: | K Forestry > K10 Forestry production F Plant production > F30 Plant genetics and breeding | ||||
Subjects: | Not in use, please see Agris categories | ||||
Agrovoc terms: | breeding methods, propagation by cuttings, selection, picea abies, somatic embryogenesis, cloning, forestry | ||||
Keywords: | breeding, cutting propagation, early selection, mass propagation, Picea abies, somatic embryogenesis | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-106 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-106 | ||||
ID Code: | 403 | ||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) | ||||
Deposited By: | Karl-Anders Högberg | ||||
Deposited On: | 20 Nov 2003 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:04 |
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