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Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce

Högberg, Karl-Anders (2003). Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Silvestria, 1401-6230 ; 294
ISBN 91-576-6528-1
[Doctoral thesis]

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Abstract

The use of vegetative mass propagation in practical forestry with Norway spruce (Picea abies (L.) Karst.) is limited at present, although its potential to deliver high genetic gains is obvious. The objective of this thesis was to study possibilities and limitations of vegetative propagation when applied in different parts of a breeding/mass propagation system for Norway spruce. Two vegetative propagation methods were studied: somatic embryogenesis and cutting propagation. Somatic embryogenesis was accompanied by losses of genotypes during the propagation process. The embryogenic response at proliferation and maturation was under family control, while germination was obtained for all families. Parental effects on proliferation and maturation were found for male parents but not for female. However, no correlations between embryogenic characters and breeding goal traits could be detected on parental level. Shortening of treatment with abscisic acid (ABA) during somatic embryo development gave pronounced positive effects on height growth of regenerated plants. An improved protocol, including five weeks ABA treatment and root development in liquid medium significantly improved performance of the resulting plants. The number of plants with lateral roots at the time of ex vitro transfer increased substantially with this protocol. Lateral roots at ex vitro transfer were shown to be a marker for good height growth and clonal uniformity during the next two years. Selection for height of cutting propagated clones in the nursery resulted in low responses in height after six years in field. The likely reason for this was low correlations between nursery traits and field traits. Genotype x environment interactions in the studied clonal test series varied from close to zero to more than 50% of the clone component. A tendency towards increased interaction components with age was obtained in one of the series. In situations with large genotype x environment interactions, clonal stability over sites should be included in the selection criteria.

Authors/Creators:Högberg, Karl-Anders
Title:Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce
Year of publishing :November 2003
Volume:294
Number of Pages:39
Papers/manuscripts:
NumberReferences
ALLI. Högberg, K.-A., Ekberg, I., Norell, L. & von Arnold, S. 1998. Integration of somatic embryogenesis in a tree breeding programme – a case study with Picea abies. Canadian journal of forest research 28: 1536-1545. II. Högberg, K.-A., Bozhkov, P. V., Grönroos, R. & von Arnold, S. 2001. Critical factors affecting ex vitro performance of somatic embryo plants of Picea abies. Scandinavian journal of forest research 16: 295-304. III. Högberg, K.-A., Bozhkov, P. V. & von Arnold, S. 2003. Early selection improves clonal performance and reduces intraclonal variation of Norway spruce plants propagated by somatic embryogenesis. Tree physiology 23: 211-216. IV. Högberg, K.-A. & Karlsson, B. 1998. Nursery selection of Picea abies clones and effects in field trials. Scandinavian journal of forest research 13: 12-20. V. Karlsson, B. & Högberg, K.-A. 1998. Genotypic parameters and clone x site interaction in clone tests of Norway spruce (Picea abies (L.) Karst.). Forest genetics 5: 21-30.
Place of Publication:Uppsala
ISBN for printed version:91-576-6528-1
ISSN:1401-6230
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:K Forestry > K10 Forestry production
F Plant production > F30 Plant genetics and breeding
Subjects:Not in use, please see Agris categories
Agrovoc terms:breeding methods, propagation by cuttings, selection, picea abies, somatic embryogenesis, cloning, forestry
Keywords:breeding, cutting propagation, early selection, mass propagation, Picea abies, somatic embryogenesis
URN:NBN:urn:nbn:se:slu:epsilon-106
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-106
ID Code:403
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Karl-Anders Högberg
Deposited On:20 Nov 2003 00:00
Metadata Last Modified:02 Dec 2014 10:04

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