Home About Browse Search
Svenska


Field attractants for Pachnoda interrupta selected by means of GC-EAD and single sensillum screening

Bengtsson, Jonas M. and Wolde-Hawariat, Yitbarek and Khbaish, Hamida and Negash, Merid and Jembere, Bekele and Seyoum, Emiru and Hansson, Bill S. and Larsson, Mattias C. and Hillbur, Ylva (2009). Field attractants for Pachnoda interrupta selected by means of GC-EAD and single sensillum screening. Journal of chemical ecology. 35:9, 1063-1076
[Journal article]

[img]
Preview
PDF
576kB

Official URL: http://www.springerlink.com/content/955355187v0u04...

Abstract

The sorghum chafer, Pachnoda interrupta Olivier (Coleoptera: Scarabaeidae: Cetoniinae), is a key pest on sorghum, Sorghum bicolor (L.) Moench (Poaceae), in Ethiopia. At present there is a lack of efficient control methods. Trapping shows promise for reduction of the pest population, but would benefit from the development of attractive lures. To find attractants that could be used for control of P. interrupta, either by mass trapping or by monitoring as part of integrated pest management, we screened headspace collections of sorghum and the highly attractive weed Abutilon figarianum Webb (Malvaceae) for antennal activity using gas chromatograph-coupled electroantennographic detection (GC-EAD). Compounds active in GC-EAD were identified by combined gas chromatography and mass spectrometry (GC-MS). Field trapping suggested that attraction is governed by a few influential compounds, rather than specific odor blends. Synthetic sorghum and abutilon odor blends were attractive, but neither blend outperformed the previously tested attractants eugenol and methyl salicylate, of which the latter was also part of the abutilon blend. The strong influence of single compounds led us to search for novel attractive compounds, and to investigate the role of individual olfactory receptor neurons (ORNs) in the perception of kairomones. We screened the response characteristics of ORNs to 82 putative kairomones in single sensillum recordings (SSR), and found a number of key ligand candidates for specific classes of ORNs. Out of these key ligand candidates, six previously untested compounds were selected for field trapping trials: anethole, benzaldehyde, racemic 2,3-butanediol, isoamylalcohol, methyl benzoate and methyl octanoate. The compounds were selected on the basis that they activated different classes of ORNs, thus allowing us to test potential kairomones activating large non-overlapping populations of the peripheral olfactory system, while avoiding redundant multiple activations of the same ORN type. Field trapping results revealed that racemic 2,3-butanediol is a powerful novel attractant for P. interrupta.

Authors/Creators:Bengtsson, Jonas M. and Wolde-Hawariat, Yitbarek and Khbaish, Hamida and Negash, Merid and Jembere, Bekele and Seyoum, Emiru and Hansson, Bill S. and Larsson, Mattias C. and Hillbur, Ylva
Title:Field attractants for Pachnoda interrupta selected by means of GC-EAD and single sensillum screening
Series/Journal:Journal of chemical ecology (0098-0331)
Year of publishing :11 September 2009
Volume:35
Number:9
Page range:1063-1076
Publisher:Springer Netherlands
ISSN:0098-0331
Language:English
Additional Information:Dr. Bekele Jembere passed away on March 26th 2009 due to sudden illness. We dedicate this article to his memory.
Publication Type:Journal article
Refereed:Yes
Full Text Status:Public
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Plant production > Plant and forest protection
Keywords:Electrophysiology, Field trapping test, Olfaction, Polyphagous herbivore, Pest control, Attractants, Pachnoda interrupta, Sorghum chafer, Coleoptera, Scarabaeidae
URN:NBN:urn:nbn:se:slu:epsilon-4-137
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-4-137
ID Code:4035
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: Jonas Bengtsson
Deposited On:12 Oct 2009 00:00
Metadata Last Modified:02 Dec 2014 10:27

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits