Lindgren, Ove
(2003).
Carotenoid Biosynthesis in Seed of Arabidopsis thaliana.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae. Agraria, 1401-6249
; 431
ISBN 91-576-6472-2
[Doctoral thesis]
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891kB |
Abstract
Seed of Arabidopsis thaliana accumulate carotenoids during development. These are mainly β-carotene, violaxanthin and lutein. By overexpression of an endogenous phytoene synthase (psy) under control of the seed-specific napin (nap) promoter the carotenoid content was elevated, resulting in increased levels in mainly β-carotene, violaxanthin, lutein and α-carotene. Transgenic seeds were also found to contain increased levels of ABA and displayed a delay in germination. The levels of ABA had a good correlation to the varying increases in lutein and violaxanthin content in seeds from plants hemizygous for the nap:psy construct. Seeds from plants overexpressing a β-carotene ketolase (bkt) from the green algae Haematococcus pluvialis, also under control of the napin promoter contained small amounts of ketocarotenoids, mainly 4-keto-lutein. When plants carrying the nap:bkt construct were crossed to plants carrying nap:psy this resulted in seeds where the total ketocarotenoid content had increased up to 13-fold. In seeds of the aba1-3 mutant of Arabidopsis which is mutated in the zeaxanthin epoxididase (zep) zeaxanthin levels were increased up to 40-fold and in seed of plants overexpressing an endogenous β-carotene hydroxylase (bch) it was found that the violaxanthin content had increased up to 20-fold. In seed from plants transgenic for nap:bch, neoxanthin and ABA levels were also increased. Just as for plants transgenic for nap:psy these seeds displayed a delay in germination. When γ-tocopherol content was analysed it was found that in seeds of nap:psy and nap:bch transformants it was only half of that found in wild type. In the Arabidopsis mutant abscisic acid-insensitive 3-1 (abi3-1) that is mutated in a seed-specific transcription factor, the seeds were found to have increased levels of β-carotene, violaxanthin, lutein and γ-tocopherol.
Authors/Creators: | Lindgren, Ove | ||||
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Title: | Carotenoid Biosynthesis in Seed of Arabidopsis thaliana | ||||
Year of publishing : | December 2003 | ||||
Number: | 431 | ||||
Number of Pages: | 33 | ||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||
ISBN for printed version: | 91-576-6472-2 | ||||
ISSN: | 1401-6249 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agris subject categories.: | X Agricola extesions > X30 Life sciences | ||||
Subjects: | Not in use, please see Agris categories | ||||
Agrovoc terms: | arabidopsis thaliana, carotenoids, aba, biosynthesis, seeds, germination | ||||
Keywords: | Arabidopsis thaliana, carotenoids, phytoene synthase, napin, ABA, β-carotene ketolase, β-carotene hydroxylase, γ-tocopherol. | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-114 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-114 | ||||
ID Code: | 426 | ||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) | ||||
Deposited By: | Ove Lindgren | ||||
Deposited On: | 11 Dec 2003 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:04 |
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