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Evaluation of sperm production, testicular measurements and post-thaw sperm quality in Tori and Estonian breed stallions

Kavak, Ants (2004). Evaluation of sperm production, testicular measurements and post-thaw sperm quality in Tori and Estonian breed stallions. Uppsala : Sveriges lantbruksuniv.
ISBN 91-576-6600-8
[Licentiate thesis]

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The present study was aimed to evaluate daily sperm output, testicular measurements and post-thaw sperm quality of Tori and Estonian breed stallions. In the first study, daily sperm output (DSO) and testicular measurements were evaluated. One ejaculate was collected daily for 10 subsequent days from 8 Tori (T) and 8 Estonian (E) breed stallions. The daily sperm output was calculated as the mean of the total sperm number (TSN) collected on days 8-10 in T stallions and on days 4-10 in E stallions. The DSO of T stallions was 12.9x109 spermatozoa and of E stallions 4.5x109 spermatozoa (p<0.001). All testicular measurements were significantly smaller in E than in T stallions (P<0.001). Testicular circumference was 45.4 cm and 35.4 cm in T and E stallions, respectively (P<0.001). The testicular circumfe-ren-ce was correlated with DSO in both E (P<0.05) and T stallions (P=0.071). In paper II, the sperm motility, membrane and acrosome integrity as well as the capacitation status of frozen-thawed spermatozoa from 7 T and 6 E stallions with CASA and flow cytometry (using a combination of the fluorophore stains SNARF, PI and FITC-PSA, and Merocyanine-540/Yo-Pro-1, respec-tively) were studied. Two ejaculates were cryopreserved from each stallion in 0.5 ml plastic straws according to the Hannover method. Two straws per ejaculate and per stallion were thawed at 37C for 30 sec. Motility was analysed with CASA immediately after thawing, whereas for flow cytometry, spermato-zoa were cleansed by 70:40 % Percoll discontinuous density gradient separation before being analysed for sperm viability, acrosome integrity (triple stain) and capacitation status (Merocyanine 540/Yo-Pro-1). Results are presented as least square means (LSmeans). The motility of frozen-thawed semen was 43.4 % for T and 42.3 % for E stallions (P>0.05). After Percoll separation, 79.3 % of the sperma-tozoa from T stallions had intact acrosomes and 1.7 % of them showed early signs of capacitation. The same parameters for E stallions were 84.5 % and 2.3 %, respectively. There were no statistically significant differences between breeds or ejaculates within breed for any evaluated parameter

Authors/Creators:Kavak, Ants
Title:Evaluation of sperm production, testicular measurements and post-thaw sperm quality in Tori and Estonian breed stallions
Year of publishing :January 2004
Number of Pages:26
ALL Kavak, A., Lundeheim, N., Aidnik, M., Einarsson, S. 2003. Testicular measurements and daily sperm output of Tori and Estonian breed stallions. Reproduction in Domestic Animals 38: 167–169 Kavak, A., Johannisson, A., Lundeheim, N., Rodriguez-Martinez, H., Aidnik, M., Einarsson, S. 2003. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry. Animal Reproduction Science 76, 205–216
Place of Publication:Uppsala
ISBN for printed version:91-576-6600-8
Publication Type:Licentiate thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
Subjects:Not in use, please see Agris categories
Agrovoc terms:stallions, testes, semen, spermatozoa, movement, cell membranes, cell counting, quality, fertility
Keywords:testicular measurement, daily sperm output, motility, plasma membrane integrity, acrosome status, capacitation status, horse
Permanent URL:
ID Code:442
Department:(VH) > Institutionen för obstetrik och gynekologi
Deposited By: Ants Kavak
Deposited On:19 Jan 2004 00:00
Metadata Last Modified:02 Dec 2014 10:04

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