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Spermadhesin PSP-I/PSP-II heterodimer induces migration of polymorphonuclear neutrophils into the uterine cavity of the sow
Rodriguez--Martinez, Heriberto and Saravia, Fernando and Wallgren, Margareta and Martinez, E.A. and Sanz, Luis and Roca, J. and Vazquez, J.M. and Calvete, Juan
(2010).
Spermadhesin PSP-I/PSP-II heterodimer induces migration of polymorphonuclear neutrophils into the uterine cavity of the sow.
Journal of reproductive immunology. 84
:2010:1
, 57-65
[Journal article]
Official URL: http://dx.doi.org/10.1016/j.jri.2009.10.007 AbstractThe heterogeneous seminal plasma (SP) is involved in endometrial inflammation and following immune changes that occur post-mating in pigs. This study tested whether SP spermadhesins (binding heparin [HBPs] or not [PSP I/PSP II]) recruit different lymphocyte subsets (CD2+, CD4+ and CD8+) and polymorphonuclear leukocytes (PMNs) to the superficial endometrium/lining epithelium and lumen, respectively, of oestrous sows, in vivo. In Experiment 1, endometrial biopsies were taken under narcosis from six sows whose uterine horns were separated and their lumen infused with 3 mg/mL spermadhesins (HBP resp PSP I/PSP II) in 100 mL saline (treatment horn) or 100 mL saline (control horn). Endometrial samples removed between 2 and 120 min post-infusion were studied by immunohistochemistry (IHC, using mABs) or histology, to assess the degree of T cell respectively PMN entry over time. In Experiment 2, eight conscious oestrous sows were singly infused intra-utero with 3 mg/mL of PSP I/PSP II in 100 mL saline (n = 4) or 100 mL of saline (n = 4), to assess relative PMN numbers in the uterine lumen, 3 h post-infusion. Compared to controls, PSP I/PSP II infusion significantly recruited uterine lymphocytes from 10 min (CD2+) or from 60 min (CD8+) onwards, while HBPs had only increased CD4+ cells by 120 min. As well, PSP I/PSP II, but not HBPs, significantly (P < 0.05) induced an early (10 min) PMN migration to the surface epithelium, 5 fold by 30 min and 7 fold from 60 min onwards (P < 0.001), with PMNs visible in the lumen from 30 min of infusion. Six fold more PMNs were collected from the uterine lumen of PSP I/PSP II-infused sows compared to controls, 3 h after infusion (P < 0.001). Seminal plasma PSP I/PSP II heterodimer triggers the entry of cleansing uterine PMNs, initiating a cascade of transient and long-lasting immunological events in oestrous sows. Authors/Creators: | Rodriguez--Martinez, Heriberto and Saravia, Fernando and Wallgren, Margareta and Martinez, E.A. and Sanz, Luis and Roca, J. and Vazquez, J.M. and Calvete, Juan |
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Title: | Spermadhesin PSP-I/PSP-II heterodimer induces migration of polymorphonuclear neutrophils into the uterine cavity of the sow |
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Series Name/Journal: | Journal of reproductive immunology |
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Year of publishing : | 2010 |
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Volume: | 84 |
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Number: | 2010:1 |
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Page range: | 57-65 |
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Publisher: | Elsevier |
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ISSN: | 0165-0378 |
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Language: | English |
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Additional Information: | Elsevier http://dx.doi.org/10.1016/j.jri.2009.10.007 |
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Publication Type: | Journal article |
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Refereed: | Yes |
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Article category: | Scientific peer reviewed |
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Version: | Accepted version |
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Full Text Status: | Public |
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Subjects: | Obsolete subject words > VETERINARY MEDICINE > Obstetrics and gynaecology |
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Keywords: | seminal plasma, spermadhesins, PMNs, inflammation, T cells, endometrium, in vivo, pig |
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URN:NBN: | urn:nbn:se:slu:epsilon-3-19 |
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Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3-19 |
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Additional ID: | Type of ID | ID |
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DOI | 10.1016/j.jri.2009.10.007 |
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ID Code: | 4550 |
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Department: | (VH) > Dept. of Clinical Sciences |
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Deposited By: |
Heriberto Rodriguez-Martinez
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Deposited On: | 03 Mar 2010 00:00 |
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Metadata Last Modified: | 02 Dec 2014 10:31 |
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Repository Staff Only: item control page Related resources Is referenced by* | Aloé, S., Weber, F., Behr, B., Sauter-Louis, C. & Zerbe, H. 2012, "Modulatory effects of bovine seminal plasma on uterine inflammatory processes", Reproduction in Domestic Animals, vol. 47, no. 1, pp. 12-19. | Is referenced by* | Caballero, I. et al. 2012, "Seminal Plasma Proteins as Modulators of the Sperm Function and Their Application in Sperm Biotechnologies", Reproduction in Domestic Animals, vol. 47, no. SUPPL.3, pp. 12-21. | Is referenced by* | Dyck, M.K., Foxcroft, G.R., Novak, S., Ruiz-Sanchez, A., Patterson, J. & Dixon, W.T. 2011, "Biological markers of boar fertility", Reproduction in Domestic Animals, vol. 46, no. SUPPL. 2, pp. 55-58. | Is referenced by* | Hansen, P.J. 2011, "The Immunology of Early Pregnancy in Farm Animals", Reproduction in Domestic Animals, vol. 46, no. SUPPL. 3, pp. 18-30. | Is referenced by* | Hunter, R.H.F., Coy, P., Gadea, J. & Rath, D. 2011, "Considerations of viscosity in the preliminaries to mammalian fertilisation", Journal of assisted reproduction and genetics, vol. 28, no. 3, pp. 191-197. | Is referenced by* | Katila, T. 2012, "Post-mating Inflammatory Responses of the Uterus", Reproduction in Domestic Animals, vol. 47, no. SUPPL. 5, pp. 31-41. | Is referenced by* | O'Leary, S., Armstrong, D.T. & Robertson, S.A. 2011, "Transforming growth factor-β (TGF) in porcine seminal plasma", Reproduction, Fertility and Development, vol. 23, no. 6, pp. 748-758. | Is referenced by* | Rodríguez-Martínez, H., Kvist, U., Ernerudh, J., Sanz, L. & Calvete, J.J. 2011, "Seminal plasma proteins: What role do they play?", American Journal of Reproductive Immunology, vol. 66, no. SUPPL. 1, pp. 11-22. | References* | Alghamdi, A.S., Lovaas, B.J., Bird, S.L., Lamb, G.C., Rendahl, A.K., Taube, P.C. & Foster, D.N. 2009, "Species-specific interaction of seminal plasma on sperm-neutrophil binding", Animal Reproduction Science, vol. 114, no. 4, pp. 331-344. | References* | Caballero, I., Vazquez, J.M., García, E.M., Parrilla, I., Roca, J., Calvete, J.J., Sanz, L. & Martínez, E.A. 2008, "Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa", Theriogenology, vol. 70, no. 8, pp. 1352 | References* | Dapino, D.G., Teijeiro, J.M., Cabada, M.O. & Marini, P.E. 2009, "Dynamics of heparin-binding proteins on boar sperm", Animal Reproduction Science, vol. 116, no. 3-4, pp. 308-317. | References* | Gerner, W., Käser, T. & Saalmüller, A. 2009, "Porcine T lymphocytes and NK cells - An update", Developmental and comparative immunology, vol. 33, no. 3, pp. 310-320. | References* | Johansson, M., Bromfield, J.J., Jasper, M.J. & Robertson, S.A. 2004, "Semen activates the female immune response during early pregnancy in mice", Immunology, vol. 112, no. 2, pp. 290-300. | References* | O'Leary, S., Jasper, M.J., Robertson, S.A. & Armstrong, D.T. 2006, "Seminal plasma regulates ovarian progesterone production, leukocyte recruitment and follicular cell responses in the pig", Reproduction, vol. 132, no. 1, pp. 147-158. | References* | Piriou-Guzylack, L. & Salmon, H. 2008, "Membrane markers of the immune cells in swine: An update", Veterinary research, vol. 39, no. 6. | References* | Robertson, S.A. 2007, "Seminal fluid signaling in the female reproductive tract: lessons from rodents and pigs.", Journal of animal science, vol. 85, no. 13 Suppl, pp. E36-44. | References* | Schuberth, H.J., Taylor, U., Zerbe, H., Waberski, D., Hunter, R. & Rath, D. 2008, "Immunological responses to semen in the female genital tract", Theriogenology, vol. 70, no. 8, pp. 1174-1181. | References* | Taylor, U., Rath, D., Zerbe, H. & Schuberth, H.-. 2008, "Interaction of intact porcine spermatozoa with epithelial cells and neutrophilic granulocytes during uterine passage", Reproduction in Domestic Animals, vol. 43, no. 2, pp. 166-175. | References* | Zenclussen, A.C. 2006, "Regulatory T cells in pregnancy", Springer seminars in immunopathology, vol. 28, no. 1, pp. 31-39. |
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