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Frost-related dieback of Swedish and Estonian Salix plantations due to pathogenic and ice nucleation-active bacteria

Cambours, Marie-Anne (2004). Frost-related dieback of Swedish and Estonian Salix plantations due to pathogenic and ice nucleation-active bacteria. Uppsala : Sveriges lantbruksuniv.
ISBN 91-576-6637-7
[Licentiate thesis]

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During the past decade, important dieback has been observed in short-rotation forestry plantations of Salix viminalis and S. dasyclados in Sweden and Estonia, plantations from which the isolation of ice nucleation-active (INA) and pathogenic bacteria has also been reported. This thesis investigates the connection between bacterial infection and frost as a possible cause for such damage, and the role played by internal and external factors (e.g. plant frost sensitivity, fertilisation) in the dieback observed. Bacterial floras isolated from ten Salix clones growing on fertilised/unfertilised mineral soil or nitrogen-rich organic soil, were studied. Culturable bacterial communities present both in internal necrotic tissues and on the plant surface (i.e. epiphytes) were isolated on two occasions (spring and autumn). The strains were biochemically characterised (with gram, oxidase and fluorescence tests), and tested for ice nucleation-activity. Their pathogenic properties were studied with and without association to a freezing stress. Certain strains were eventually identified with BIOLOG plates and 16S rRNA analysis. A high number of culturable bacterial strains was found in the plant samplings, belonging mainly to Erwinia and Sphingomonas spp.; pathogenic and INA communities being mostly Erwinia-, Sphingomonas- and Xanthomonas–like. The generally higher plant dieback noted in the field on nutrient-rich soils and for frost sensitive clones was found connected to higher numbers of pathogenic and INA bacteria in the plants. We thus confirm Salix dieback to be related to a synergistic effect of frost and bacterial infection, possibly aggravated by fertilisation.

Authors/Creators:Cambours, Marie-Anne
Title:Frost-related dieback of Swedish and Estonian Salix plantations due to pathogenic and ice nucleation-active bacteria
Year of publishing :August 2004
Number of Pages:33
ALLI.Cambours M-A., Nejad P., Granhall U. & Ramstedt M. 2004. Frost-related dieback of willows. Comparison of epiphytically and endophytically isolated bacteria from different Salix clones, with emphasis on ice nucleation activity, pathogenic properties and seasonal variation. Biomass and bioenergy (In press). II.Cambours M-A., Heinsoo K., Granhall U., & Nejad P. Frost-related dieback in Estonian energy plantations of willows in relation to fertilisation and pathogenic bacteria. (Submitted).
Place of Publication:Uppsala
ISBN for printed version:91-576-6637-7
Publication Type:Licentiate thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
F Plant production > F62 Plant physiology - Growth and development
Subjects:Not in use, please see Agris categories
Agrovoc terms:forest plantations, dieback, salix, frost damage, erwinia, pathogenicity, estonia, sweden
Keywords:willow, freezing damage, ice formation, pathogenicity, frost resistance, fertiliser, Sphingomonas, Erwinia
Permanent URL:
ID Code:597
Department:(NL, NJ) > Enheten för växtpatologi och biologisk bekämpning (fr.o.m. 990101)
Deposited By: Marie-Anne Cambours
Deposited On:17 Aug 2004 00:00
Metadata Last Modified:02 Dec 2014 10:05

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