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Studies of articular cartilage macromolecules in the equine middle carpal joint, in joint pathology and training

Skiöldebrand, Eva (2004). Studies of articular cartilage macromolecules in the equine middle carpal joint, in joint pathology and training. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Veterinaria, 1401-6257 ; 176
ISBN 91-576-6651-2
[Doctoral thesis]

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Abstract

Lameness caused by osteoarthritis (OA) is the most common reason for failure to race among horses. Degenerative changes to the articular cartilage are commonly seen often with subchondral bone sclerosis. Cartilage is a connective tissue with tensile strength and resilience, consisting of cells and abundant extracellular matrix. The cells are adapting the matrix to environmental conditions by synthesis and degradation of the matrix structural elements. A change in the concentration of macromolecules in synovial fluid and serum can indicate early biochemical changes in the joint. This provides a tool for research into and monitoring pathogenic mechanisms of OA. The content of COMP, aggrecan and collagen type II was measured in serum and synovial fluid (sf) from trotters and riding horses with either normal joints or joints with different stages of cartilage pathology. The trotters with a training background and cartilage degeneration had low concentrations of COMP and aggrecan in synovial fluid and serum as well as sf-collagen (paper I). The effect of long-term training on the concentration of sf- COMP, -aggrecan and -collagen type II from young trotters was measured (paper II). The amount of training and age influenced sf-COMP, where lower concentrations were related to more training and higher age. The concentration of collagen type II degradation products increased with total days of training. In vitro dynamic compression of cartilage explants from trained horses showed a down-regulation of COMP synthesis compared to untrained horses (paper II). COMP in cartilage matrix was presented in an ultrastructural study (paper III), where COMP concentration, was much less abundant in loaded and unloaded matrix of articular cartilage from a strenuously trained horse compared to untrained horses. The untrained horses often displayed a higher immunolabeling in loaded areas compared to unloaded areas, indicating that dynamic load can promote COMP synthesis and /or retention, while excessive load have opposite effect. Galloping horses with osteochondral fractures had higher concentration of sf-COMP compared to normal or osteoarthtritic joints from trotters or galloping horses (paper IV). In conclusion the present work shows that strenuously training of racehorses alter the expression of macromolecules in the articular cartilage of the third carpal bone.

Authors/Creators:Skiöldebrand, Eva
Title:Studies of articular cartilage macromolecules in the equine middle carpal joint, in joint pathology and training
Year of publishing :September 2004
Volume:176
Number of Pages:54
Papers/manuscripts:
NumberReferences
ALLI. Skiöldebrand, E., Lorenzo, P., Zunino, L., Rucklidge, G. J., Sandgren B., Carlsten, J., & Ekman, S. (2001) Concentration of collagen, aggrecan and cartilage oligomeric matrix protein (COMP) in synovial fluid from equine middle carpal joints. Equine Vet J 33, 394-402. II. Skiöldebrand, E., Heinegård, D., Wong, M., Siegrist, M., Önnerfjord, P., Olofsson, B., Rucklidge, G.J., Roneus, N. & Ekman, S. Altered homeostasis of extracellular matrix proteins in articular cartilage of intensely training Standardbred trotters. Manuscript submitted for publication. III. Skiöldebrand, E., Ekman, S., Idsund, E., Heinegård, D. & Hultenby, K. Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in loaded and unloaded areas of the articular cartilage from the equine middle carpal joint. In manuscript. IV. Skiöldebrand, E., Heinegård, D., Eloranta, M-J., Nilsson, G., Dudhia, J., Sandgren, B. & Ekman, S. (2004) Enhanced concentration of cartilage oligomeric matrix protein (COMP) in osteochondral fractures from racing Thoroughbreds. Journal of Orthopaedic Research. In press.
Place of Publication:Uppsala
ISBN for printed version:91-576-6651-2
ISSN:1401-6257
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L74 Miscellaneous animal disorders
Subjects:Not in use, please see Agris categories
Agrovoc terms:joint diseases, collagen, synovial fluid, training, fractures, joints (body parts), horses
Keywords:COMP, aggrecan, collagen type II, osteoarthritis, synovial fluid, training, dynamic compression, osteochondral fractures, middle carpal joint, horses
URN:NBN:urn:nbn:se:slu:epsilon-310
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-310
ID Code:641
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Staff Epsilon
Deposited On:23 Sep 2004 00:00
Metadata Last Modified:02 Dec 2014 10:06

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