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Biological control of grey mould in Swedish forest nurseries

Capieau, Kristof (2004). Biological control of grey mould in Swedish forest nurseries. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., ; 325
ISBN 91-576-6709-8
[Doctoral thesis]

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Abstract

Grey mould, caused by Botrytis cinerea Pers.:Fr., is a severe problem in conifer seedling production in Swedish forest nurseries. The overall aim of the study was to investigate the possibility of biological control and to find naturally occurring needle fungi with biosuppressive activity against grey mould on conifer seedlings in Swedish forest nurseries. The potential of biological control was demonstrated by means of the microbial products Binab, GlioMix and Mycostop. These products reduced B. cinerea infections in Scots pine seedlings by 16 to 57% in a field trial at a Swedish forest nursery, and by 51 to 94% in growth room assays under controlled climatic conditions. The effect of BinabÒ and GlioMixÒ was of the same level as the fungicide tolylfluanid. A novel microcosm bioassay was developed to evaluate microorganisms for biocontrol of B. cinerea in co-inoculation tests on three-week-old Scots pine (Pinus sylvestris L.) seedlings. Pre-inoculation treatment by incubation at 40 °C for five hours predisposed seedlings to infection by B. cinerea. Different isolates of needle fungi were low to highly effective in suppression of B. cinerea. One of these isolates was identified as a Fusarium sp. strain F31 and was shown to secrete a spectrum of enniatins in liquid medium. Two new enniatins, J2 and J3, were co-isolated. Inhibitory activity to spore germination of B. cinerea was shown for the enniatins B, B1, B4 and K1. Based on the screening tests, nine fungal isolates were selected and evaluated for their efficacy to suppress spontaneous infections by B. cinerea in first-year Scots pine seedlings in a small-scale field experiment. Two isolates of Trichoderma sp. and the fungicide tolylfluanid showed some degree of control. The impact of the tested control agents on the community structure of needle-inhabiting fungi was analysed. This thesis describes the first steps in the development of biological control of grey mould in forest nurseries. Further investigations are needed in the search for an efficient biocontrol method that can replace or complement chemical control.

Authors/Creators:Capieau, Kristof
Title:Biological control of grey mould in Swedish forest nurseries
Year of publishing :September 2004
Volume:325
Number of Pages:37
Papers/manuscripts:
NumberReferences
ALLI. Capieau K., Stenlid, J. & Stenström, E. 2004. Potential for biological control of Botrytis cinerea in Pinus sylvestris seedlings. Scandinavian Journal of Forest Research, 19, 312-319. II. Capieau K., Stenlid, J. & Stenström, E. Application of a novel bioassay to screen microorganisms for biocontrol of grey mould on Scots pine seedlings. (Manuscript) III. Pohanka, A., Capieau, K., Broberg, A., Stenlid, J., Stenström, E. & Kenne, L. 2004. Enniatins of Fusarium sp. strain F31 and their inhibition of Botrytis cinerea spore germination. Journal of Natural Products, 67, 851-857. IV. Capieau K., Stenlid, J. & Stenström, E. The effect of antagonistic fungi and a fungicide on the incidence of Botrytis cinerea Pers.:Fr. and the community structure of needle-inhabiting fungi in Pinus sylvestris L. seedlings. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:91-576-6709-8
ISSN:1401- 6230
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
Subjects:Not in use, please see Agris categories
Agrovoc terms:antibiotics, secondary metabolites, botrytis cinerea, moulds, fungal diseases, biological control, microscopy, coniferous forests
Keywords:antibiotics, antifungal secondary metabolites, Botryotinia fuckeliana, depsipeptides, gray mold, scanning electron microscopy
URN:NBN:urn:nbn:se:slu:epsilon-316
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-316
ID Code:646
Department:(S) > Institutionen för skoglig mykologi och patologi
Deposited By: Kristof Capieau
Deposited On:27 Sep 2004 00:00
Metadata Last Modified:02 Dec 2014 10:06

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