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Seed-borne fungi of the afromontane tree species Podocarpus falcatus and Prunus africana in Ethiopia

Gure, Abdella (2004). Seed-borne fungi of the afromontane tree species Podocarpus falcatus and Prunus africana in Ethiopia. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Silvestria, 1401-6230 ; 334
ISBN 91-576-6718-7
[Doctoral thesis]

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Abstract

This thesis is comprised of four studies regarding seed-borne fungi of the afromontane forest trees, Podocarpus falcatus (Thunb. Mirb.) and Prunus africana (Hook. F.) Kalkman, in Ethiopia. Based on morphology and molecular data from the rDNA (ITS) region, a diverse group of mainly Ascomycota, some Basidiomycota and a few Zygomycota were identified. Phylogenetic analysis of the ITS sequences revealed several clades differentiated according to the host. Some of these fungi were previously reported as seed-borne pathogens from other parts of the world. More fungi were isolated from P. falcatus than from P. africana. Four Botryosphaeria spp., three of them from P. falcatus and one from P. africana were identified. The Botryosphaeria sp. from P. africana was a previously undescribed species and named Diplodia rosulata sp. nov. One of the three Botryosphaeria spp. from P. falcatus was identified as B. parva while the other two were previously undescribed Botryosphaeria spp. with Diplodia and Dothiorella anamorphs. Botryosphaeria parva was previously identified as a major cause of Botryosphaeria stem canker on Eucalyptus plantations in Ethiopia and elsewhere. The implications of the occurrence of B. parva both on native and exotic tree species were discussed. Based on seed and seedling inoculation tests on P. falcatus, five categories of fungi ranging from strong pathogens to germination promoters were identified. Among the tested fungi, Fusarium oxysporum and Polyporus sp. were strongly pathogenic and caused both seed rotting and seedling damping-off, while Diaporthe sp. Po21 and Diaporthe sp. Po84 promoted seed germination. Dual culture tests among seed-borne fungi of P. falcatus on 2 % malt extract agar resulted in deadlock or replacement interactions. Co-inoculation of seeds with two germination promoters and six pathogenic isolates resulted in improved germination in some of the combinations. Bioassay tests with HPLC fractions of culture filtrates of the two germination promoting Diaporthe spp. indicated antifungal activity against F. oxysporum and Ulocladium chartarum. From these studies it was concluded that different groups of fungi could be associated with seeds of forest trees. There are still many undescribed fungal species that may impact forest establishment and development. Therefore, more investigations are necessary in these areas.

Authors/Creators:Gure, Abdella
Title:Seed-borne fungi of the afromontane tree species Podocarpus falcatus and Prunus africana in Ethiopia
Year of publishing :December 2004
Volume:334
Number of Pages:31
Papers/manuscripts:
NumberReferences
ALLI. Gure A., Wahlström, K., Barklund, P. & Stenlid, J. Fungi Associated with Seeds of Afromontane Podocarpus falcatus and Prunus africana in Ethiopia. (Submitted manuscript). II. Gure, A., Slippers, B., & Stenlid, J. Seed-borne Botryosphaeria spp. from native Prunus and Podocarpus trees in Ethiopia, with a description of the anamorph Diplodia rosulata sp. nov. (Submitted manuscript). III. A. Gure, K. Wahlström and J. Stenlid. Pathogenicity of seed-associated fungi to Podocarpus falcatus in vitro. Forest Pathology. In press. IV. A. Gure and J. Stenlid. In vitro interactions among seed-borne fungi of Podocarpus falcatus. (Submitted manuscript).
Place of Publication:Uppsala
ISBN for printed version:91-576-6718-7
ISSN:1401-6230
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
Subjects:Not in use, please see Agris categories
Agrovoc terms:fungal morphology, podocarpus, prunus, botryosphaeria, pathogens, fusarium oxysporum, diaporthe, seeds, forest trees, ethiopia
Keywords:Seed-borne fungi, Botryosphaeria, Seed-borne pathogens, Fusarium oxysporum, Diaporthe sp., afromontane forest trees, Podocarpus falcatus, Prunus africana, interspecific interactions
URN:NBN:urn:nbn:se:slu:epsilon-367
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-367
ID Code:705
Department:(S) > Institutionen för skoglig mykologi och patologi
Deposited By: Abdella Gure
Deposited On:01 Dec 2004 00:00
Metadata Last Modified:02 Dec 2014 10:06

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