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Analytical and nutritional aspects of folate in cereals

Johansson, Madelene (2005). Analytical and nutritional aspects of folate in cereals. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:7
ISBN 91-576-7006-4
[Doctoral thesis]

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Abstract

A good folate status before conception and during early pregnancy protects against child birth defects, particularly neural tube defects. According to dietary surveys, only a few percent of Swedish women reach the recommended daily intake of folate (400 g). Swedish authorities are therefore discussing the introduction of mandatory folic acid fortification. A prerequisite for a decision is access to reliable data on folate in cereals since cereal food is both a major contributor to dietary folate intake and a common vehicle for fortification. Despite much progress in development of HPLC methods for folate, there is still a need for improved selectivity when analysing cereal samples with low level of folate. Ten silica-based stationary phases were compared and alkyl-bonded phases were found to be best for the separation of individual folate forms in terms of selectivity and peak shape. Best selectivity was achieved on an Aquasil C18 column. Optimising the sample purification prior to quantification can also improve the HPLC method. Reversed-phase sorbents, e.g. phenyl-endcapped and cyclohexyl-endcapped, were successfully used to purify folate from food extracts. By combining anion-exchange and reversed-phase sorbent, clean extracts of complex food samples were obtained. Several gluten-free products were analysed for folate content after deconjugation with rat serum using a validated HPLC method. Trienzyme treatment was found to be unnecessary for these samples. Gluten-free products contain considerably less folate than their gluten-containing counterparts and should be considered for folic acid fortification. Studies on stability of added folic acid during the baking procedure showed an overage of approximately 20% to be necessary to achieve targeted levels. Another important issue in deciding the appropriate fortification level is the extent to which the added folate is absorbed. In a short-term human study using volunteers with ileostomy, mean apparent absorption from folic acid-fortified bread was estimated to be 80%. In a 3-month intervention study, it was observed that a daily additional amount of 166 µg folic acid from fortified white bread was sufficient to significantly increase the folate status in women. Based on these two human studies it is concluded that bread is a suitable vehicle for folic acid fortification.

Authors/Creators:Johansson, Madelene
Title:Analytical and nutritional aspects of folate in cereals
Year of publishing :2005
Volume:2005:7
Number of Pages:63
Papers/manuscripts:
NumberReferences
ALLI. Johansson, M., Jastrebova, J., Grahn, A. and Jägerstad, M. Separation of dietary folates by reversed-phase high performance liquid chromatography: comparison of conventional and alternative silica based stationary phases. Submitted II. Nilsson, C., Johansson M., Yazynina, E., Strålsjö, L. and Jastrebova, J. (2004) Solid-phase extraction for HPLC analysis of dietary folates. European Food Research and Technology 219: 199-204 III. Yazynina, E., Jastrebova, J., Johansson, M. and Jägerstad M. Folates in gluten-free cereals and starch products. Manuscript to be submitted IV. Witthöft, C. M., Arkbåge, K., Johansson, M., Lundin, E., Berglund, G., Zhang, J-X., Lennernäs, H. and Dainty J. Folate absorption from folate-fortified and processed foods using a human ileostomy model. Submitted V. Johansson, M., Witthöft, C. M., Bruce, Å. and Jägerstad M. (2002) Study of wheat breakfast rolls fortified with folic acid. The effect on folate status in women during a 3-month intervention. European Journal of Nutrition 41: 279-286
Place of Publication:Uppsala
ISBN for printed version:91-576-7006-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:S Human nutrition > S20 Physiology of human nutrition
Subjects:Not in use, please see Agris categories
Agrovoc terms:cereals, folic acid, purification, absorption, gluten, hypersensitivity, soft wheat, nutritional requirements, pregnancy, human nutrition
Keywords:folic acid, 5-CH3-H4folate, HPLC, SPE purification, enzyme treatment, folate absorption, fortification, gluten-free products, wheat breakfast rolls
URN:NBN:urn:nbn:se:slu:epsilon-531
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-531
ID Code:757
Department:(NL, NJ) > Dept. of Food Science (until 161231)
Deposited By: Madelene Johansson
Deposited On:10 Feb 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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