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Metabolic studies in cattle using the hyperinsulinemic euglycemic clamp technique

Sternbauer, Karin (2005). Metabolic studies in cattle using the hyperinsulinemic euglycemic clamp technique. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:38
ISBN 91-576-7037-4
[Doctoral thesis]

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Abstract

Hyperinsulinemic euglycemic clamp, HEC, originally developed for determing insulin-mediated glucose disposal and insulin sensitivity in humans, was used and evaluated in cattle. In the present study the following investigations were performed: the bovine physiological responses during drug-induced insulin resistance, the influence of liberal or restricted concentrate feeding on insulin sensitivity, the impact of two different physical activity levels on insulin sensitivity and muscle characteristics, and finally the expression of abomasal efflux rates in relation to different blood glucose levels. Clenbuterol and flumethasone treatment induced insulin resistance in calves lasting less than 17 hrs and 3 days post-treatment, respectively. The effect of flumethasone was more manifest than that of clenbuterol. Insulin sensitivity and muscle characteristics did not differ between one group of calves exposed to light exercise and another group of calves not exercised for 4 to 5 weeks. The results indicated that growth may have an influence on insulin sensitivity. The blood glucose levels of heifers fed concentrate at the upper limit, according to Swedish feeding standards, were higher than in heifers fed quantities at the lower limit. There was no change in insulin sensitivity or insulin levels between the two groups. A high plasma glucose level was shown to reduce the abomasal efflux rate and increase the abomasal pH in non-pregnant, non-lactating cows. The results show that the HEC technique may be a useful means for investigating drug-induced insulin resistance and can also be used to create specific experimental conditions. Ruminant gluconeogenesis may still occur to some extent during HEC application, especially in lactating cows, and therefore this must be considered in future studies.

Authors/Creators:Sternbauer, Karin
Title:Metabolic studies in cattle using the hyperinsulinemic euglycemic clamp technique
Year of publishing :2005
Volume:2005:38
Number of Pages:31
Papers/manuscripts:
NumberReferences
ALLI. Sternbauer, K., Luthman, J., Hänni, A. and Jacobsson, SO. (1998) Clenbuterol-induced insulin resistance in calves measured by hyperinsulinemic euglycemic clamp technique. Acta Veterinaria Scandinavica 39, 281-289. II. Sternbauer, K. and Luthman, J. (1998) Flumethasone-induced insulin resistance in calves. Journal of Veterinary Medicine, Series A 45, 441-443. III. Sternbauer, K. and Essén-Gustavsson, B. (2002) Insulin sensitivity and muscle characteristics in calves at different levels of physical activity. Journal of Veterinary Medicine, Series A 49, 449-454. IV. Sternbauer, K. and Luthman, J. (2002) Insulin sensitivity in heifers on different diets. Acta Veterinaria Scandinavica 43, 107-114. V. Holtenius, K., Sternbauer, K. and Holtenius, P. (2000) The effect of the plasma glucose level on the abomasal function in dairy cows. Journal of Animal Science 78, 1930-1935.
Place of Publication:Uppsala
ISBN for printed version:91-576-7037-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L50 Animal physiology and biochemistry
Subjects:Not in use, please see Agris categories
Agrovoc terms:insulin, glucose, clenbuterol, muscles, cattle, methods
Keywords:insulin, glucose, insulin resistance, muscle characteristics, cattle
URN:NBN:urn:nbn:se:slu:epsilon-660
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-660
ID Code:796
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Karin Sternbauer
Deposited On:17 May 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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